Human Granzyme B ELISPOT Kit (Reagents for 20 x 96 tests, without plates) (ab48706)


  • 製品名Human Granzyme B ELISPOT Kit (Reagents for 20 x 96 tests, without plates)
    Granzyme B キット 製品一覧
  • テスト
    20 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Cell culture extracts
  • アッセイタイプSandwich (qualitative)
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, oncology, infectious diseases, autoimmune diseases and transplantation.

    This Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.


    After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Coloured "purple" spots indicate cytokine production by individual cells.

  • アプリケーション適用あり: ELISpotmore details
  • 試験プラットフォームReagents


  • 関連性Cytolytic T lymphocytes and natural killer cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.
  • 細胞内局在Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells
  • 別名
    • C11
    • CGL1
    • CSPB
    • CTLA1
    • Fragmentin 2
    • Granzyme 2
    • SECT
    see all
  • 参照データベース


Our Abpromise guarantee covers the use of ab48706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISpot Use at an assay dependent dilution.


Human Granzyme B ELISPOT Kit (Reagents for 20 x 96 tests, without plates) (ab48706) 使用論文

ab48706 has not yet been referenced specifically in any publications.

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