製品の概要

  • 製品名
    Human Granzyme B ELISA Kit (CTLA-1)
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    A 15 356pg/ml 18 5.06%
    B 15 73pg/ml 5 7.38%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    A 8 536pg/ml 30 5.59%
    B 8 138pg/ml 9 6.76%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 20 pg/ml
  • 検出範囲
    31.25 pg/ml - 1000 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 73 31.25pg/ml - 1000pg/ml

  • 全工程の試験時間
    3h 45m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Granzyme B (CTLA-1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Granzyme B in Human serum, plasma, buffered solutions and cell culture medium.

    A monoclonal antibody specific Granzyme B has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Granzyme B concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Granzyme B are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Granzyme B present in the samples.

    This kit will recognize both endogenous and recombinant Human Granzyme B.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 ラベル 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-Granzyme B Red 1 x 400µl 2 x 400µl
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    Granzyme B Microplate (12 x 8 well strips) 1 unit 2 units
    Granzyme B Standard (Lyophilized) Yellow 2 vials 4 vials
    HRP Diluent Red 1 x 23ml 1 x 23ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • 研究分野
  • 関連性
    The lytic enzymes of cytotoxic lymphocytes seem to be contained within lysosome-like granules found in the cytoplasm. These granules contain perforin, a membrane pore-forming protein, and a family of serine proteases (granzymes). Granzyme B, a 32 kDa protein, is a serine Aspase associated with the granules of Natural Killer (NK) cells. Granzyme B seems to be able to elicit cell death, perhaps by activating an endogenous pathway of programmed cell death.
  • 細胞内局在
    Cytoplasmic
  • 別名
    • C11
    • CTLA 1
    • Fragmentin 2
    • Granzyme 2
    • HLP
    • SECT
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab46142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Hu Granzyme B (CTLA-1) measured in biologicals showing quantity (pg) per mL of tested sample

  •  Representative Standard Curve using ab46142

プロトコール

参考文献

This product has been referenced in:
  • Scholz S  et al. Human hantavirus infection elicits pronounced redistribution of mononuclear phagocytes in peripheral blood and airways. PLoS Pathog 13:e1006462 (2017). ELISA ; Human . Read more (PubMed: 28640917) »
  • Patel SA  et al. Mesenchymal stem cells protect breast cancer cells through regulatory T cells: role of mesenchymal stem cell-derived TGF-beta. J Immunol 184:5885-94 (2010). Human . Read more (PubMed: 20382885) »

See all 4 Publications for this product

レビューと Q&A

Thank you for contacting us.

Antibodies included in the Granzyme kit are raised in mouse. The capture antibody coated onto the wells is (Fab)' 2 IgG1, and the biotiinylated detection antibody tracer is whole IgG1, and final concentration is ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録