製品の概要

  • 製品名Human Glucokinase ELISA Kit (GCK)
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 5.1%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.4%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    1.5 ng/ml
  • 検出範囲
    1.563 ng/ml - 50 ng/ml
  • 添加回収試験

    96 %

  • 全工程の試験時間
    4h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Glucokinase (GCK) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human GCK in plasma, serum, and cell culture supernatants.

    A Glucokinase specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Glucokinase specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Glucokinase captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage.
  • 組織特異性Isoform 1 is expressed in pancreas. Isoform 2 and isoform 3 is expressed in liver.
  • 関連疾患Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) [MIM:125851]; also shortened MODY-2. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
    Defects in GCK are the cause of familial hyperinsulinemic hypoglycemia type 3 (HHF3) [MIM:602485]; also known as persistent hyperinsulinemic hypoglycemia of infancy (PHHI) or congenital hyperinsulinism. HHF is the most common cause of persistent hypoglycemia in infancy. Unless early and aggressive intervention is undertaken, brain damage from recurrent episodes of hypoglycemia may occur.
  • 配列類似性Belongs to the hexokinase family.
  • Information by UniProt
  • 別名
    • ATP D hexose 6 phosphotransferase
    • FGQTL3
    • GCK
    • GK
    • GLK
    • Glucokinase
    • Glucokinase (hexokinase 4)
    • hexokinase 4
    • hexokinase D
    • hexokinase D, pancreatic isozyme
    • Hexokinase type IV
    • Hexokinase-4
    • Hexokinase-D
    • HHF3
    • HK IV
    • HK4
    • HKIV
    • HXK4_HUMAN
    • HXKP
    • LGLK
    • Liver Glucokinase
    • MODY2
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab125967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Glucokinase ELISA Kit (GCK) 画像

  • Representative Standard Curve using ab125967

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Human Glucokinase ELISA Kit (GCK) (ab125967) 使用論文

ab125967 has not yet been referenced specifically in any publications.

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