製品の概要

  • 製品名
    Human GITR Ligand ELISA Kit (TNFSF18)
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 25 pg/ml
  • 検出範囲
    41 pg/ml - 10000 pg/ml
  • 添加回収試験

    97 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 87.83 67% - 100%
    Serum 116.9 108% - 123%
    Plasma 87.69 67% - 100%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s GITR Ligand Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human GITR Ligand in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for Human GITR Ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and GITR Ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human GITR Ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GITR Ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

  • 機能
    Cytokine that binds to TNFRSF18/AITR/GITR. Important for interactions between activated T-lymphocytes and endothelial cells and may modulate T-lymphocyte survival in peripheral tissues.
  • 組織特異性
    Expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells.
  • 配列類似性
    Belongs to the tumor necrosis factor family.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 別名
    • Activation inducible TNF related ligand
    • Activation-inducible TNF-related ligand
    • AITR ligand
    • AITRL
    • GITR ligand
    • GITRL
    • Glucocorticoid induced TNF related ligand
    • Glucocorticoid induced TNFR related protein ligand
    • Glucocorticoid-induced TNF-related ligand
    • hGITRL
    • MGC138237
    • TL6
    • TNF18_HUMAN
    • TNFSF18
    • TNLG2A
    • Tumor necrosis factor (ligand) superfamily member 18
    • Tumor necrosis factor ligand 2A
    • Tumor necrosis factor ligand superfamily member 18
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab113323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human GITR Ligand ELISA Kit (TNFSF18) 画像

  • Representative standard curve using ab113323

プロトコール

Human GITR Ligand ELISA Kit (TNFSF18) (ab113323) 使用論文

ab113323 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab113323.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録