製品の概要

  • 製品名Human GITR Ligand ELISA Kit (TNFSF18)
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 25 pg/ml
  • 検出範囲
    41 pg/ml - 10000 pg/ml
  • 添加回収試験

    97 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 87.83 67% - 100%
    Serum 116.9 108% - 123%
    Plasma 87.69 67% - 100%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s GITR Ligand Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human GITR Ligand in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human GITR Ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and GITR Ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human GITR Ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GITR Ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab113323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human GITR Ligand ELISA Kit (TNFSF18) 画像

  • Representative standard curve using ab113323

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Human GITR Ligand ELISA Kit (TNFSF18) (ab113323) 使用論文

ab113323 has not yet been referenced specifically in any publications.

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