Human GDNF ELISA Kit (Glial Derived Neurotrophic Factor) (ab100525)


  • 製品名
    Human GDNF ELISA Kit (Glial Derived Neurotrophic Factor)
    GDNF キット 製品一覧
  • 検出方法
  • サンプルの種類
    Cell culture supernatant, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 4 pg/ml
  • 検出範囲
    2.74 pg/ml - 2000 pg/ml
  • 添加回収試験

    100 %

    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 119.8 85% - 140%
    Plasma 99.25 86% - 113%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s GDNF (Glial Derived Neurotrophic Factor) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human GDNF in plasma and cell culture supernatants. (Human GDNF concentration is pretty low in normal plasma, it may not be detected in this assay).

    This assay employs an antibody specific for Human GDNF coated on a 96-well plate. Standards and samples are pipetted into the wells and GDNF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human GDNF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GDNF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    We have not been able to detect the endogenous Human GDNF in normal serum with ab100525, only in serum spiked with Human GDNF.

  • 特記事項

    Optimization may be required with urine samples

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム



Our Abpromise guarantee covers the use of ab100525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.


  • Representative standard curve using ab100525

  • HuGDNF measured in biological fluids showing quantity (pg) per mL of tested sample

  • Representative standard curve using ab100525



This product has been referenced in:
  • Zhang Y  et al. The protein and mRNA expression levels of glial cell line-derived neurotrophic factor in post stroke depression and major depressive disorder. Sci Rep 7:8674 (2017). Read more (PubMed: 28819313) »

See 1 Publication for this product

レビューと Q&A

Excellent Kit

Good Good 4/5 (Ease of Use)
We used the kit to determine GDNF in neuroblastoma cell line culture supernatant. We followed the protocol provided in the kit and we were able to successfully detect the levels of GDNF.

Abcam user community

Verified customer

投稿 Jan 24 2018

The phenol red within the cell medium should not interfere with the measurement at 450nm but we would suggest running a media control just to make sure which can also act as your negative control.

With regards to the sample preparation, n...

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Thank you for contacting us. We do not currently sell any ELISA kits that are specifically designed for use on rat samples. After checking with the lab, our mouse and human ELISA kits for these targets will be unlikely to cross-react with rat, but have...

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Thank you for your email which has been forwarded to me inthe technical support department.

I am happy to confirm that these kits should not be affected in their performanceif the packagewas not directly placed in therefrigeratorand thatthey ...

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Vielen Dank für Ihre Email und Ihren Anruf heute morgen.

Ich kann Ihnen bestätigen, dass beide Kits, ab99978 und ab100525, ohne Problem einen Tag bei Raumtemperatur aushalten können. Die Puffer und Antikörper können d...

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Thank you for your inquiry.

Unfortunately, we do not know of any publications thatreferenced product at the moment. We will up-date the datasheet assoon as we receive any information about publications using ab100525.

I am sorry to ...

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