Human GC Globulin ELISA Kit (Vitamin D Binding Protein) (ab108853)

製品の概要

  • 製品名
    Human GC Globulin ELISA Kit (Vitamin D Binding Protein)
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 3.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 9.1%
  • サンプルの種類
    Serum, Plasma
  • アッセイタイプ
    Competitive
  • 検出感度
    > 0.05 µg/ml
  • 検出範囲
    0.098 µg/ml - 100 µg/ml
  • 添加回収試験

    97 %

  • 全工程の試験時間
    3h 00m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Human GC Globulin (Vitamin D Binding Protein) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitive measurement of GC Globulin in plasma, serum, saliva, milk and cell-culture supernatants.

    A GC Globulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated GC Globulin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of GC Globulin captured in plate.

  • アプリケーション
    適用あり: Competitive ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Competitive ELISA Use at an assay dependent dilution.

画像

  • Representative Standard Curve using ab108853.

プロトコール

参考文献

ab108853 has not yet been referenced specifically in any publications.

レビューと Q&A

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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