Human GAPDH exon 1 ChIP Primer and Probe Set (ab85378)

製品の概要

  • 製品名
    Human GAPDH exon 1 ChIP Primer and Probe Set
    GAPDH キット 製品一覧
  • 製品の概要

    Accession number: NM_002046.
    The HsGAPDH primers and probe can be used to quantify a control locus in chromatin immunoprecipitation (ChIP) assays with chromatin prepared from human cells.

    GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) is a highly expressed housekeeping gene. The primers and probe are located in exon 1, within 1 kb of the transcription start site. In most cell types, this locus is highly enriched with histone modifications associated with active gene transcription such as Histone H3 K9 acetylation (see image with ab4441). Whereas it shows low levels of histone modifications associated with silencing such as Histone H3 K9 tri methylation (see image with ab71604).

製品の特性

参考文献

ab85378 has not yet been referenced specifically in any publications.

レビューと Q&A

I can confirm thatany of the loci we use for positive and negative controlsin the ChIP image on the datasheet would be good to use.In this case for example, we expect a strong signal in the active loci (GAPDH, RPL30 and ALDOA) and very weak signal in t...

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I have confirmed with our laboratory that the primer pairs were specifically generated for the human sequences.  We have not tested them with mouse and therefore cannot confirm that they give a single, specific PCR product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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