製品の概要

  • 製品名Human G-CSF ELISA Kit
    G-CSF キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 1 pg/ml
  • 検出範囲
    0.69 pg/ml - 500 pg/ml
  • 添加回収試験

    > 75 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 76.56 68% - 90%
    Serum 83.45 72% - 94%
    Plasma 74.76 67% - 87%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s G-CSF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human G-CSF in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human G-CSF coated on a 96-well plate. Standards and samples are pipetted into the wells and G-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human G-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of G-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Save time with a 90-minute ELISA protocol. Try Human G-CSF ELISA Kit (CSF3) (ab188390) from our SimpleStep ELISA® range.

  • 特記事項

    Optimization may be required with urine samples

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性Granulocyte Colony Stimulating Factor (G-CSF) a cytokine that controls the production, differentiation, and function of granulocytes. It is a potent stimulator of bone marrow cells especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils. Recombinant human G-CSF has been developed by pharmaceutical companies, and since the late 1980's, multiple clinical trials have explored its efficacy in a variety of conditions.
  • 別名
    • C17orf33
    • Colony stimulating factor 3 (granulocyte)
    • CSF 3
    • CSF beta
    • CSF3
    • CSF3OS
    • Csfg
    • Filgrastim
    • GCSA
    • GCSF
    • Granulocyte colony stimulating fact
    • Lenograstim
    • Macrophage granulocyte inducer 2
    • MGC45931
    • MGI 2
    • MGI IG
    • OTTMUSP00000006785
    • Pluripoietin
    • RP23-395E10.4
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab100524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human G-CSF ELISA Kit 画像

  • Standard curve in dilutent B with background signal subtracted (duplicates; +/- SD).

  • Human G-CSF measured in undiluted culture supernatants (duplicates +/- SD).

  • Representative standard curve using ab100524

  • G-CSF measured in undiluted human plasma, signal in serum was below level of detection (duplicates +/- SD).

  • Representative standard curve using ab100524

プロトコール

Human G-CSF ELISA Kit (ab100524) 使用論文

ab100524 has not yet been referenced specifically in any publications.

Product Wall

We have not tested the GCSF ELISA kit with Cynomolgus monkey serum. However, when looking at the human GCSF sequence our kit recognizes and testing the cross-reactivity to the Cynomolgus sequence, there are a number of predicted matches, including gran...

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The standard recombinant protein of ab100524 was expressed in E.coli.

The standard we use for this kit (ab100524) is recombinant human G-CSF: 18.7 kDa protein; 174 amino acid residues. We have not tested this kit with a met-GCSF, but the following amino acid sequence of the recombinant standard used should help the custo...

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Thank you for contacting us.

Serum can be stored at -80C for a month, this will be having minimal affect on the assay.

The following publication will help understanding the affect of temperature on different serum protein.

ht...

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Thank you for your reply.

The strips come with a rack where the single strips can be inserted.

This rack will fit all standard plate readers.

I am sorry, I cannot check if this is also true for your plate reader. Please as...

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Thank you for your inquiry.

I can confirm that with ab100524 it is not necessary to use the whole plate at one time.

The plate comes in convenient 12 strips x 8 wells.

Please follow the instruction of the protocol when sto...

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Thank you for contacting Abcam.

We still have not tested whether the kit can be used to quantitate PEGfilgrastim, therefore the original response, "to spike in pegylated G-CSF and see" is still the only option for answering this que...

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Thank you for your inquiry.  That is a good question.  I reviewed our testing records on ab100524 but unfortunately I was not able to find any data about pegylated G-CSF.  Therefore we don’t know if this kit will also detect the pegylated form....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"