Human Furin ELISA Kit (ab113322)
Key features and details
- Sensitivity: 15 pg/ml
- Range: 13.72 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Furin ELISA Kit
Furin キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 15 pg/ml -
検出範囲
13.72 pg/ml - 10000 pg/ml -
添加回収試験
84 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 89.57 80% - 98% Plasma 77.74 69% - 85% Cell culture media 84.69 75% - 93% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Furin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Furin in serum, plasma (collect plasma using heparin and EDTA as an anticoagulant. Citrate is not recommended) and cell culture supernatants.
This assay employs an antibody specific for Human Furin coated on a 96-well plate. Standards and samples are pipetted into the wells and Furin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Furin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Furin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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特記事項
Optimization may be required with urine samples.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human Furin Item F 2 vials Furin Microplate (12 x 8 wells) Item A 1 unit Recombinant Human Furin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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参照データベース
- Entrez Gene: 5045 Human
- SwissProt: P09958 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab113322 は 1 報の論文で使用されています。
- Kaur G et al. Persistently Increased Systemic ACE2 Activity Is Associated With an Increased Inflammatory Response in Smokers With COVID-19. Front Physiol 12:653045 (2021). PubMed: 34122129