- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Forkhead box P3
Forkhead box protein P3
Immune dysregulation polyendocrinopathy enteropathy X linked
Immunodeficiency polyendocrinopathy enteropathy X linked
Probable transcription factor. Plays a critical role in the control of immune response.
Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
All lanes : Anti-FOXP3 antibody (ab10901) at 0.4 µg/ml
Lane 1 : 20µg HEK 293 lysate Lane 2 : 20µg HEK 293 lysate over expressing human FOXP3 Lane 3 : 20µg HEK 293 lysate over expressing mouse FOXP3 Lane 4 : 20µg HEK 293 lysate with Human FOXP3 peptide (ab16809) at 1 µg/ml Lane 5 : 20µg HEK 293 lysate over expressing human FOXP3 with Human FOXP3 peptide (ab16809) at 1 µg/ml Lane 6 : 20µg HEK 293 lysate over expressing mouse FOXP3 with Human FOXP3 peptide (ab16809) at 1 µg/ml