Human Follistatin ELISA Kit (ab113319)

製品の概要

  • 製品名Human Follistatin ELISA Kit
    Follistatin キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 0.5 ng/ml
  • 検出範囲
    0.512 ng/ml - 125 ng/ml
  • 添加回収試験

    90 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 107.3 102% - 113%
    Serum 86.98 81% - 92%
    Plasma 75.78 67% - 83%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Follistatin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Follistatin in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for Human Follistatin coated on a 96-well plate. Standards and samples are pipetted into the wells and Follistatin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Follistatin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Follistatin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Save time with a 90-minute ELISA protocol. Try Human Follistatin ELISA Kit (ab192147) from our SimpleStep ELISA® range.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab113319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Follistatin ELISA Kit 画像

  • Representative standard curve using ab113319

プロトコール

Human Follistatin ELISA Kit (ab113319) 使用論文

ab113319 has not yet been referenced specifically in any publications.

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Abreviews
We trialed the Follistatin Human ELISA kit for Mouse Serum Samples to determine its effectiveness in detecting mouse follistatin. The kit was performed according to Abcam's protocol and guidelines. We found that the Follistatin Human ELISA kit is not effective in detecting mouse follistatin. While the human standards demonstrated a good standard curve, the mouse standards, which were created using recombinant mouse follistatin (R&D Systems), did not show much difference between the maximum and minimum concentrations. Additionally, there was a precipitate that formed in the mouse standards that was not identifiable and interfered with the absorbance reading.
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Verified customer

投稿 Nov 11 2014

Thank you for your enquiry. I am sorry it has taken some time to confirm this information for you. I can confirm that heparin-plasma is compatible with ab113319 Follistatin Human ELISA Kit. The kit is compatible with heparin, EDTA, and citrate anti...

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Thank you for contacting us.  The Follistatin ELISA kit ab113319 is compatible with heparin, EDTA, and citrate anti-coagulants.  I hope this helps, please let me know if you need any additional information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"