製品の概要

  • 製品名
    Human Flt3 Ligand ELISA Kit
    Flt3 ligand キット 製品一覧
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 1.5 pg/ml
  • 検出範囲
    4.1 pg/ml - 400 pg/ml
  • 添加回収試験

    95 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 97.62 85% - 108%
    Serum 95.48 84% - 107%
    Plasma 94.39 83% - 106%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Flt3 Ligand Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Flt3 Ligand in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Flt3 Ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and Flt3 Ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Flt3 Ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Flt3 Ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab100521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab100521.

  • Representative Standard Curve using ab100521.

プロトコール

参考文献

ab100521 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you very much for sending the details. These were very helpful.

As we discussed on phone please try the experiment again by keeping all reagents at room temperature.

Please let me know if you need further assistance.

Thank you very much for your phone call this morning.

As discussed I will be followingthis case further. Could you please provide the following details;

- Types of sample used. If supernatant, what type of cells were incubated
- D...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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