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|Cell culture supernatant||97.62||85% - 108%|
|Serum||95.48||84% - 107%|
|Plasma||94.39||83% - 106%|
Abcam’s Flt3 Ligand Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Flt3 Ligand in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human Flt3 Ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and Flt3 Ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Flt3 Ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Flt3 Ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples
|内容||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|600X HRP-Streptavidin Concentrate||1 x 200µl|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-Human Flt3 Ligand||2 vials|
|Flt3 Ligand Microplate (12 x 8 wells)||1 unit|
|Recombinant Human Flt3 Ligand Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100521 has not yet been referenced specifically in any publications.