製品の概要

  • 製品名Human FLRG ELISA Kit
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 250 pg/ml
  • 検出範囲
    204.8 pg/ml - 50000 pg/ml
  • 添加回収試験

    89 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 94.6 67% - 130%
    Serum 95.75 84% - 103%
    Plasma 77.86 67% - 99%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s FLRG Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human FLRG coated on a 96-well plate. Standards and samples are pipetted into the wells and FLRG present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FLRG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FLRG bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab113318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human FLRG ELISA Kit 画像

  • Representative standard curve using ab113318

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Human FLRG ELISA Kit (ab113318) 使用論文

ab113318 has not yet been referenced specifically in any publications.

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