Human Fibronectin ELISA Kit (ab108848)


  • 製品名Human Fibronectin ELISA Kit
    Fibronectin キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    サンプル N 平均値 SD CV%
    Overall 4.2%
    サンプル N 平均値 SD CV%
    Overall 8%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Serum, Plasma
  • アッセイタイプCompetitive
  • 検出感度
    = 0.7 µg/ml
  • 検出範囲
    0.78 µg/ml - 50 µg/ml
  • 添加回収試験

    98 %

  • 全工程の試験時間
    3h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Fibronectin Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fibronectin levels in plasma and serum.

    A Fibronectin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Fibronectin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Fibronectin captured in plate.

  • アプリケーション適用あり: Competitive ELISAmore details
  • 試験プラットフォームMicroplate


  • 機能Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • 組織特異性Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
  • 関連疾患Glomerulopathy with fibronectin deposits 2
  • 配列類似性Contains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.
  • 発生段階Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
  • 翻訳後修飾Sulfated.
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylated by FAM20C in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  • 細胞内局在Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • 別名
    • CIG
    • Cold insoluble globulin
    • Cold-insoluble globulin
    • DKFZp686F10164
    • DKFZp686H0342
    • DKFZp686I1370
    • DKFZp686O13149
    • ED B
    • Fibronectin 1
    • FINC
    • FN
    • FN1
    • FNZ
    • GFND
    • GFND2
    • LETS
    • Migration stimulating factor
    • MSF
    • Ugl-Y3
    see all
  • 参照データベース


Our Abpromise guarantee covers the use of ab108848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Competitive ELISA Use at an assay dependent concentration.

Human Fibronectin ELISA Kit 画像

  • Fibronectin measured in various samples showing quantity (microgram) per mL of tested sample

  • Representative Standard Curve using ab108848.


Human Fibronectin ELISA Kit (ab108848) 使用論文

ab108848 has not yet been referenced specifically in any publications.

Product Wall

Very easy ELISA, just a few hours and you'll have data

Excellent Excellent 5/5 (Ease of Use)
Used citrate plasma samples which were diluted 1:100 as suggested in the protocol which I followed from start to finish.
Standard curve not quite straight in the higher concentrations, so appropriate sample dilution is important.

Very easy to use kit. Will buy it again.

Abcam user community

Verified customer

投稿 Oct 28 2014

I can confirm that this kit theoretically recognizes both form of fibronectin.

The insoluble cellular form will only be bound once by the antibody since they are monoclonal. Since fibronectin is then assembled into an insoluble matri...

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Abreview for Fibronectin Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)

Sample: Human Serum (Human plasma)
Specification: Human plasma
Amount of Sample: 2.5 µg
Type of Kit Used: ELISA
Positive control: recombinant fibronectin
Did you use the Abcam Kit protocol?: Yes

Abcam user community

Verified customer

投稿 Jan 15 2013

Thank you very much for your interest our products.

To our knowledge, ab108848 has not been tested in urine. However by participating in our AbTrial program you can now use our products in an untested application or species without financial...

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Siento no haber contestado antes, me temo que no tengo la información de todos los productos solicitados, sin embargo, puedo proporcionarte acerca de los siguientes kits:

El kit PLTP Activity Fluorometric Assay Kit (ab65386) no es apro...

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Thank you for your query.

I assume you customer 's kit has the lot number 1211713 (GR68088-1)?

I can confirm that the standard is HumanFibronectinin a buffered protein base (250 mg, lyophilized).

It should be reconstituted...

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