製品の概要

  • 製品名Human FGF6 ELISA Kit
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 6 pg/ml
  • 検出範囲
    8.23 pg/ml - 6000 pg/ml
  • 添加回収試験

    95 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 95.42 84% - 103%
    Serum 95.13 83% - 103%
    Plasma 94.63 82% - 102%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s FGF6 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF6 in serum, plasma (collect plasma using EDTA as an anticoagulant. Heparin and citrate are not recommended. Human FGF6 concentration is pretty low in normal serum/plasma, it may not be detected in this assay), and cell culture supernatants.

    This assay employs an antibody specific for Human FGF6 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF6 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm.

  • 特記事項

    Optimization may be required with urine samples

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性FGF6 is a secreted heparin binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF6 is expressed in leukemia cell lines with platelet megakaryocytic differentiation potential. It signals through FGFR 1c, 2c, and 4.
  • 別名
    • FGF 6
    • Fibroblast growth factor 6
    • Fibroblast growth factor 6 precursor
    • HBGF 6
    • HBGF6
    • Heparin binding growth factor 6
    • Heparin secretory transforming protein 2
    • HST 2
    • HST2
    • HSTF 2
    • HSTF2
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human FGF6 ELISA Kit 画像

  • Representative Standard Curve using ab100518.

  • Representative Standard Curve using ab100518.

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Human FGF6 ELISA Kit (ab100518) 使用論文

ab100518 has not yet been referenced specifically in any publications.

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