製品の概要

  • 製品名Human FGF4 ELISA Kit
    FGF4 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture extracts, Tissue Extracts
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 50 pg/ml
  • 検出範囲
    24.69 pg/ml - 18000 pg/ml
  • 添加回収試験

    92 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture extracts 93.45 94% - 103%
    Tissue Extracts 91.96 82% - 102%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s FGF4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF4 in cell lysates and tissue lysates.

    This assay employs an antibody specific for Human FGF4 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性FGF4 is a heparin binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF4 signals through the FGFR 1c, 2c, 3c, and 4.
  • 別名
    • Fibroblast growth factor 4
    • HBGF4
    • Heparin binding growth factor 4
    • Heparin secretory transforming protein 1
    • HST
    • HST1
    • HSTF1
    • KS3
    • Transforming protein KS3
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human FGF4 ELISA Kit 画像

  • Representative standard curve using ab100517

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Human FGF4 ELISA Kit (ab100517) 使用論文

ab100517 has not yet been referenced specifically in any publications.

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