製品の概要

  • 製品名Human Fetuin A ELISA Kit (AHSG)
    Fetuin A キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 10 pg/ml
  • 検出範囲
    312 pg/ml - 20000 pg/ml
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Human Fetuin A (AHSG) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Fetuin A (AHSG) in cell culture supernatants, serum and plasma (heparin, EDTA).

    A Fetuin A (AHSG) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Fetuin A (AHSG) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Fetuin A (AHSG) amount of sample captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.
  • 組織特異性Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins.
  • 配列類似性Belongs to the fetuin family.
    Contains 2 cystatin fetuin-A-type domains.
  • 翻訳後修飾Phosphorylation sites are present in the extracelllular medium.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • A2HS
    • AHS
    • Ahsg
    • Alpha 2 HS glycoprotein
    • Alpha-2-HS-glycoprotein chain B
    • Alpha-2-Z-globulin
    • Ba-alpha-2-glycoprotein
    • FETUA
    • FETUA_HUMAN
    • Fetuin-A
    • HSGA
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Fetuin A ELISA Kit (AHSG) 画像

  • Representative Standard Curve using ab119594

プロトコール

Human Fetuin A ELISA Kit (AHSG) (ab119594) 使用論文

ab119594 has not yet been referenced specifically in any publications.

Product Wall

ab119594 has not been tested with mouse samples, and we do not recommend it for use with mouse samples. We also do not recommend it's use with rat samples either.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"