製品の概要

  • 製品名Human Fetuin A ELISA Kit (AHSG)
    Alpha 2 HS Glycoprotein キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 5.1%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.1%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    > 3 ng/ml
  • 検出範囲
    3.13 ng/ml - 200 ng/ml
  • 添加回収試験

    = 99 %

  • 全工程の試験時間
    4h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Fetuin A (AHSG) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fetuin A concentrations in Human plasma, serum, urine, milk and cell culture supernatants.


    A Fetuin A specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fetuin A specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fetuin A captured in plate.


    For higher sensitivity in only 90 minutes, we recommend Human Fetuin A ELISA Kit (AHSG) (ab185989) from our SimpleStep ELISA® range.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab108855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

Human Fetuin A ELISA Kit (AHSG) 画像

  • Representative Standard Curve using ab108855.

プロトコール

Human Fetuin A ELISA Kit (AHSG) (ab108855) 使用論文

This product has been referenced in:
  • Abdullah MI  et al. Tissue and serum samples of patients with papillary thyroid cancer with and without benign background demonstrate different altered expression of proteins. PeerJ 4:e2450 (2016). Sandwich ELISA ; Human . Read more (PubMed: 27672505) »
  • Májek P  et al. Plasma proteome changes associated with refractory anemia and refractory anemia with ringed sideroblasts in patients with myelodysplastic syndrome. Proteome Sci 11:14 (2013). Read more (PubMed: 23566303) »

See all 3 Publications for this product

Product Wall

Thank you for your patience, I apologize for the delay in my reply. The lab has confirmed that this kit will not cross-react with bovine alpha 2 HS Glycoprotein, and that FBS will not interfere with your results. I hope this helps, please let me know i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"