製品の概要

  • 製品名Human Ferritin ELISA Kit
    Ferritin キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Control 48 < 5.4%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Control 48 < 6.1%
  • テスト
    1 x 96 test
  • サンプルの種類
    Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    0.53 ng/ml
  • 添加回収試験

    98.66 %

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Ferritin competitive Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Ferritin in serum and plasma.

     

    A 96-well plate has been precoated with anti-Ferritin IgG antibodies. Samples, standards and the Ferritin-HRP conjugate are added to the wells, where Ferritin in the sample and standards binds to the precoated antibody and added Ferritin-HRP conjugate binds to this antibody-AFP complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Ferritin in the sample and the intensity is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 保存方法Store at +4°C. Please refer to protocols.
  • 内容 ラベル 1 x 96 tests
    10X Washing Solution 1 x 50ml
    Anti-Ferritin IgG Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit
    Anti-Ferritin-HRP conjugate 1 x 12ml
    Cover foil 1 unit
    Ferritin Control 1 x 1ml
    Ferritin Standard 0 - 0 ng/mL 1 x 3ml
    Ferritin Standard 1 - 5 ng/mL 1 x 1ml
    Ferritin Standard 2 - 20 ng/mL 1 x 1ml
    Ferritin Standard 3 - 100 ng/mL 1 x 1ml
    Ferritin Standard 4 - 400 ng/mL 1 x 1ml
    Ferritin Standard 5 - 1,000 ng/mL 1 x 1ml
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution 1 x 15ml
  • 研究分野
  • 機能Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
  • 関連疾患Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
    Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.
  • 配列類似性Belongs to the ferritin family.
    Contains 1 ferritin-like diiron domain.
  • Information by UniProt
  • 別名
    • ferritin L chain
    • Ferritin L subunit
    • Ferritin light chain
    • ferritin light polypeptide
    • ferritin light polypeptide like 3
    • FRIL_HUMAN
    • FTL
    • NBIA3
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab108698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

Human Ferritin ELISA Kit 画像

  • Representative Standard Curve using ab108698

プロトコール

Human Ferritin ELISA Kit (ab108698) 使用論文

ab108698 has not yet been referenced specifically in any publications.

Product Wall

ab108698 Ferritin Human ELISA Kit: for pre-treatment of serum samples we recommend HEPES buffer.

ab108837 Ferritin Human ELISA KIt: I am sorry this is not tested with other buffers, so we would not be able to advise which buffer to use. Ther...

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The c-Kit Human ELISA Kit (ab45924) and Ferritin Human ELISA Kit (ab108698) both come with strip wells provided.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"