製品の概要

  • 製品名Human Fas Ligand ELISPOT Kit
    Fas Ligand キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    5 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Cell culture extracts
  • アッセイタイプSandwich (qualitative)
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Intended use

     

    The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.

    The Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

     

    Principle of the method

     

    After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells

     

     

    Recognizes natural human Fas ligand

  • 特記事項

    Store plates at room temperature.

  • アプリケーション適用あり: ELISpotmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性The protein encoded by this gene is the ligand for FAS. Both are transmembrane proteins. Interaction of FAS with Fas Ligand is critical in triggering apoptosis of some types of cells such as lymphocytes. Defects in this gene may be related to some cases of systemic lupus erythematosus.
  • 細胞内局在Cell membrane; single-pass type II membrane protein. Secreted protein. May be released into the extracellular fluid, probably by cleavage form the cell surface.
  • 別名
    • APT1LG1
    • APTL
    • CD95L
    • CD95L protein
    • Fas antigen ligand
    • FASL
    • FASLG
    • TNFSF6
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab46605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISpot Use at an assay dependent dilution.

Human Fas Ligand ELISPOT Kit (ab46605) 使用論文

ab46605 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"