製品の概要

  • 製品名Human Fas Ligand ELISA Set (without plates)
  • テスト
    5 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 44 pg/ml
  • 検出範囲
    62.5 pg/ml - 2000 pg/ml
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Fas Ligand (Fas-L) Human in vitro ELISA Set is designed for the quantitative measurement of Fas-L in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    The Fas L ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    This ELISA Set is intended to measure Fas L in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

     

    SPECIFICITY: Recognizes both natural and recombinant soluble human Fas-Ligand RANGE: 62.5pg/ml - 2000pg/ml SENSITIVITY: <12pg/ml

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームReagents

製品の特性

  • 関連性Fas / APO1 (CD95) is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane mediated apoptosis. Ligation of Fas by Fas Ligand or an anti Fas cross linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas Ligand system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumors. Tumor cells frequently exhibit de novo expression of Fas Ligand, which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas Ligand system. The full length of FasL contains 281 amino acids, consisting of the transmembrane domain, extracellular domain, and the cytoplasmic region. A metalloproteinase cleaves the membrane-bound FasL to produce soluble Fas (sFasL), which exists as a homotrimer. The sFasL shows lower cytotoxicity than membrane bound FasL.
  • 細胞内局在Cell membrane: single pass type II membrane protein. Secreted protein: may be released into the extracellular fluid, probably by cleavage form the cell surface.
  • 別名
    • Apoptosis (APO 1) antigen ligand 1
    • APT1LG1
    • APTL
    • CD178
    • CD178 antigen
    • CD95 ligand
    • CD95L protein
    • FAS antigen ligand
    • Fas L
    • Fasl Fas ligand (TNF superfamily member 6)
    • FASLG
    • Generalized lymphoproliferative disease
    • Gld
    • TNFSF6
    • Tumor necrosis factor (ligand) superfamily member 6
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab83703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Fas Ligand ELISA Set (without plates) 画像

  • Fas Ligand standard curve ranging from 62.5 to 2000 pg/mL

Human Fas Ligand ELISA Set (without plates) (ab83703) 使用論文

ab83703 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"