Human Fas Ligand ELISA Kit (APTL) (ab100515)

製品の概要

  • 製品名Human Fas Ligand ELISA Kit (APTL)
    Fas Ligand キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 2 pg/ml
  • 検出範囲
    1.37 pg/ml - 1000 pg/ml
  • 添加回収試験

    75 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 76.99 70% - 90%
    Serum 76.36 70% - 87%
    Plasma 73.42 67% - 86%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Fas Ligand (APTL) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Fas Ligand in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Fas Ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and Fas Ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Fas Ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fas Ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性Fas / APO1 (CD95) is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane-mediated apoptosis. Ligation of Fas by Fas Ligand or an anti Fas cross linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas Ligand system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumours. Tumour cells frequently exhibit de novo expression of Fas Ligand, which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas Ligand system.
  • 細胞内局在Cell Membrane (single-pass type II membrane protein) and Secreted
  • 別名
    • Apoptosis (APO 1) antigen ligand 1
    • Apoptosis antigen ligand
    • APT1LG1
    • APTL
    • CD178
    • CD95 L
    • CD95 ligand
    • CD95L
    • CD95L protein
    • Fas antigen ligand
    • FASL
    • FASLG
    • TNF superfamily member 6
    • TNFSF6
    • Tumor necrosis factor (ligand) superfamily member 6
    • Tumor necrosis factor ligand superfamily member 6
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Fas Ligand ELISA Kit (APTL) 画像

  • Representative Standard Curve using ab100515.

  • Representative Standard Curve using ab100515.

プロトコール

Human Fas Ligand ELISA Kit (APTL) (ab100515) 使用論文

ab100515 has not yet been referenced specifically in any publications.

Product Wall

good working Elisa

Good Excellent 5/5 (Ease of Use)
Abreviews
This is a good Elisa.
The results are good and reproducible.
The protocol booklet is well explained and easy to understand and easy to perform.
I have only used 1 kit for measuring Fas-Ligand so far.
I used this kit for serum samples from a comparison of healthy donor and atopic patients.
The kit is easy to use and quick compared to other Sandwich Elisa.
Based on the recommendations within the booklet we used a serum dilutions of 1: 100 and these dilutions was within the linear range for all but of the 40 samples.
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Abcam user community

Verified customer

投稿 Apr 08 2015

We would suggest testing a range of dilutions to find a dilution within the standard curve. A good range is neat, 1:5, 1:10, 1:20. In the protocol the suggested dilution for normal serum/plasma: 2-10 fold, again this needs to be optimized.

Unfortunately, the OD standard is never twice the same, therefore, it is so important to run a fresh standard curve for EVERY assay. The OD needs to be measured, it cannot be not calculated.
How to analyze the data is described under section 8, pag...

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The exact epitopes are not known but the antibody pair is binding somewhere on the extracellular domain (aa 134-281). If it helps, the capture antibody is monoclonal and the detection antibody is polyclonal. We do not have a kit against HA although we ...

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I can confirm that these kits will detect both soluble and membrane bound Fas and fas ligand.

In order to prevent membrane bound protein to interfere with your results, I suggest to spin down the saliva samples to get rid of all cells while t...

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I have reviewed the details on the datasheets for these kits. For the Fas ligand kits:

ab45892 Fas Ligand Human ELISA Kit
ab100515 Fas Ligand Human ELISA Kit

Sensitivities are different:ab100515 < 2 pg/ml, ab45892 < 12 pg/ml ...

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These ELISA kits are from two different sources but they are similar.

I recommend to determine which is the best for your needs from the datasheet and protocol information. Please take not of the different ranges these kits are suitable for. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"