製品の概要

  • 製品名Human FAS ELISA Kit (CD95)
    CD95 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 3 pg/ml
  • 検出範囲
    31.2 pg/ml - 2000 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 31.2pg/ml - 2000pg/ml
    Other biological fluids 31.2pg/ml - 2000pg/ml

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Human FAS (CD95) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human FAS (CD95) in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

    A FAS (CD95) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for FAS (CD95) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human FAS (CD95) amount of sample captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
  • 組織特異性Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
  • 関連疾患Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
  • 配列類似性Contains 1 death domain.
    Contains 3 TNFR-Cys repeats.
  • ドメインContains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
  • 細胞内局在Secreted and Cell membrane.
  • Information by UniProt
  • 別名
    • ALPS1A
    • APO 1
    • APO 1 cell surface antigen
    • Apo-1 antigen
    • APO1
    • Apo1 antigen
    • Apoptosis antigen 1
    • Apoptosis mediating surface antigen FAS
    • Apoptosis-mediating surface antigen FAS
    • APT1
    • CD95
    • CD95 antigen
    • Fas
    • Fas AMA
    • Fas antigen
    • FAS1
    • FASLG receptor
    • FASTM
    • TNF receptor superfamily member 6
    • TNFRSF6
    • TNR6_HUMAN
    • Tumor necrosis factor receptor superfamily member 6
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human FAS ELISA Kit (CD95) 画像

  • Representative Standard Curve using ab119571.

プロトコール

Human FAS ELISA Kit (CD95) (ab119571) 使用論文

ab119571 has not yet been referenced specifically in any publications.

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The best kit will depend on the amount of FAS (CD95) that you are expecting to detect in your samples. The kit ab100513 produces a standard curve that is linear for lower concentrations of FAS (CD95) than ab119571...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"