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|Cell culture media||100||99% - 100%|
|Extraction Buffer||101||100% - 103%|
Focal Adhesion Kinase (FAK) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Focal Adhesion Kinase (FAK) protein in cell and tissue extracts.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm
Focal Adhesion Kinase (FAK) is a non-receptor protein-tyrosine kinase that is implicated in signaling pathways involved in cell motility, proliferation and apoptosis. FAK is widely expressed in adult tissues as well as being critical during development (angiogenesis, heart and nervous system development). Post-translational modifications include phosphorylation at residue Tyrosine 397. Phosphorylation at this residue promotes interaction with SRC family members and signal transduction (including activation of AKT, MAPK and Rho GTPase cascades); dephosphorylation at this residue promotes focal adhesion disassembly. FAK overexpression and increased kinase activity is observed in many types of cancer.
|内容||1 x 96 tests|
|10X FAK Capture Antibody||1 x 600µl|
|10X FAK Detector Antibody||1 x 600µl|
|10X Wash Buffer PT (ab206977)||1 x 20ml|
|200X SDS||1 x 1ml|
|50X Cell Extraction Enhancer Solution (ab193971)||1 x 1ml|
|5X Cell Extraction Buffer PTR (ab193970)||1 x 10ml|
|Antibody Diluent 5BI||1 x 6ml|
|FAK Human Lyophilized Recombinant Protein||2 vials|
|Plate Seals||1 unit|
|Sample Diluent NS (ab193972)||1 x 12ml|
|SimpleStep Pre-Coated 96-Well Microplate (ab206978)||1 unit|
|Stop Solution||1 x 12ml|
|TMB Substrate||1 x 12ml|
Our Abpromise guarantee covers the use of ab187395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Background-subtracted data values (mean +/- SD) are graphed.
Background-subtracted data values (mean +/- SD, n = 2) are graphed.
Interpolated values of FAK are plotted for the indicated cell lines based on an extract load of 125 µg/mL.
The concentrations of FAK protein were interpolated from the FAK standard curve and corrected for sample dilution relative to extract load of 62.5 µg/mL. The mean FAK concentration was determined to be 44 ng/mL in HeLa extract at 62.5 µg/mL.
ab187395 has not yet been referenced specifically in any publications.