製品の概要

  • 製品名Human Factor XIII ELISA Kit
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.5%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 8.7%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 2.1 ng/ml
  • 検出範囲
    5 ng/ml - 80 ng/ml
  • 添加回収試験

    99 %

  • 全工程の試験時間
    4h 0m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Factor XIII Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of factor XIII in plasma, serum, milk, urine, saliva, and cell culture supernatants.

    A Factor XIII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor XIII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor XIII captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
  • 関連疾患Factor XIII subunit A deficiency
  • 配列類似性Belongs to the transglutaminase superfamily. Transglutaminase family.
  • 翻訳後修飾The activation peptide is released by thrombin.
  • 細胞内局在Cytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.
  • Information by UniProt
  • 別名
    • Coagulation factor XIII A chain
    • Coagulation factor XIIIa
    • F13A_HUMAN
    • F13a1
    • Protein-glutamine gamma-glutamyltransferase A chain
    • Transglutaminase A chain
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

Human Factor XIII ELISA Kit 画像

  • Representative Standard Curve using ab108836

プロトコール

Human Factor XIII ELISA Kit (ab108836) 使用論文

ab108836 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

The kit using different antibodies, but the binding site is not mapped. Since they both polyclonal antibody, it may have multiple binding sites for protein.


I hope this information is helpful to you. ...

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Thank you for contacting us.


Yes, the kit will detect both the A and B forms.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thanks for your enquiry!

Please contact our bulk order specialist, Andrea Gray at mailto:Andrea.Gray@abcam.com, regarding discounts on bulk order purchases.

I hope this is helpful. Please contact me again if you have any further que...

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Thank you for your call last week and for your patience while I've been in touch with the lab regarding your questions.

I've heard back from my contact at the lab, and she agrees that the OD values are lower than expected. An OD of 1.5-2.0 wo...

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Thank you for your call the other day.

I am happy to confirm that the kit ab108836 against the human Factor XIII will recognize the full length protein as well as the fragment of the 37 first amino acids. I have just received the confirmation...

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This ELISA kit will recognize factor XIIIa and factor XIII.  I hope this is helpful. Please contact me again if you have any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"