製品の概要

  • 製品名Human Factor XII ELISA Kit
    Factor XII キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 5%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.1%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Milk, Urine, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    0.02 ng/ml
  • 検出範囲
    6.25 ng/ml - 100 ng/ml
  • 添加回収試験

    98 %

  • 全工程の試験時間
    4h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Factor XII Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor XII concentrations in plasma, serum, milk, urine and cell culture supernatants.


    A Factor XII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor XII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor XII captured in plate.


    Save time with a 90-minute ELISA protocol. Try Human Factor XII ELISA Kit (ab192144) from our SimpleStep ELISA® range.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa.
  • 関連疾患Factor XII deficiency
    Hereditary angioedema 3
  • 配列類似性Belongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 fibronectin type-I domain.
    Contains 1 fibronectin type-II domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • 翻訳後修飾Factor XII is activated by kallikrein in alpha-factor XIIa, which is then further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of the NH2-terminal heavy chain (Coagulation factor XIIa heavy chain) and the COOH-terminal light chain (Coagulation factor XIIa light chain), connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, a light chain (Beta-factor XIIa part 2), corresponding to the COOH-terminal light chain (Coagulation factor XIIa light chain) and a nonapeptide (Beta-factor XIIa part 1).
    O- and N-glycosylated. The O-linked polysaccharides were not identified, but are probably the mucin type linked to GalNAc.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • Beta-factor XIIa part 1
    • Beta-factor XIIa part 2
    • Coagulation factor XII
    • Coagulation factor XIIa heavy chain
    • Coagulation factor XIIa light chain
    • F12
    • FA12_HUMAN
    • HAF
    • Hageman factor
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab108835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

Human Factor XII ELISA Kit 画像

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Factor XII measured in undiluted culture supernatants, MCF-7 levels below level of detection (duplicates +/- SD).

  • Factor XII measured in biological fluids (duplicates +/- SD). Human plasma and serum were tested in the dilution range of 1/100-1/4000; milk, saliva, CSF and urine were diluted 1/1-1/300.

  • Representative Standard Curve using ab108835.

プロトコール

Human Factor XII ELISA Kit (ab108835) 使用論文

ab108835 has not yet been referenced specifically in any publications.

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