製品の概要

  • 製品名
    Human Factor XI ELISA Kit
    Factor XI キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Overall 4.5%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Overall 9.8%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    = 1.3 ng/ml
  • 検出範囲
    6.25 ng/ml - 25 ng/ml
  • 添加回収試験

    96 %

  • 全工程の試験時間
    4h 00m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Factor XI Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor XI levels in plasma, serum, and cell culture supernatants.

    A Factor XI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor XI specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor XI captured in plate.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent M Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human Factor XI Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Factor XI Microplate (12 x 8 well strips) 1 unit
    Factor XI Standard 2 vials
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • 研究分野
  • 機能
    Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX.
  • 組織特異性
    Isoform 2 is produced by platelets and megakaryocytes but absent from other blood cells.
  • 関連疾患
    Factor XI deficiency (FA11D) [MIM:612416]: A hemorrhagic disease characterized by reduced levels and activity of factor XI resulting in moderate bleeding symptoms, usually occurring after trauma or surgery. Patients usually do not present spontaneous bleeding but women can present with menorrhagia. Hemorrhages are usually moderate. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • 配列類似性
    Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
    Contains 4 apple domains.
    Contains 1 peptidase S1 domain.
  • 翻訳後修飾
    Activated by factor XIIa (or XII), which cleaves each polypeptide after Arg-387 into the light chain, which contains the active site, and the heavy chain, which associates with high molecular weight (HMW) kininogen.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 別名
    • Coagulation factor XI
    • Coagulation factor XIa light chain
    • F11
    • FA11_HUMAN
    • FXI
    • Plasma thromboplastin antecedent
    • PTA
    see all
  • 参照データベース

    アプリケーション

    Our Abpromise guarantee covers the use of ab108834 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Sandwich ELISA Use at an assay dependent dilution.

    画像

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab108834.

    プロトコール

    参考文献

    ab108834 has not yet been referenced specifically in any publications.

    ab108834 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    3 reviews or Q&A

    10
    Votes: Highest First

    Dear Josephine



    Thanks for your kindly reply, this customer also wants to know the retention period of the “Factor XI standard”. Could you please help this customer to solve the problem?



    Thanks f...

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    Thank you for contacting us.

    1. At the power form, it should be good for around 1 year.
    2. After dissolve in buffer, the standard need to be aliquot and store at -80 degree if possible. If store at -80, the standard can be good for at le...

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    Product code: 108834

    Inquiry: Dear technical support team: This customer raised an enquire about ab108834 (Factor XI Human ELISA Kit), he wants to know the retention period of the “Factor XI Microplate (12 strips of 8 wells)”, co...

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    Thank you for contacting us.

    The plate can last for at least one year from the date received the kit as long as the pouch un-open. If the pouch has been opened, please keep it in the desicator if you have one. Keep dry is very important. If y...

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    Merci de votre réponse. Toutefois elle me donne une information partielle. Ma question était " combien de dosage de facteur XI différés, dans le temps, peut on faire avec un seul Kit".? Aujourd'hui je sais que le kit n'est pas utilisable plus que deux ...

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    Comme indiqué dans le protocol du kit ELISA facteur XI ab108834 (http://www.abcam.com/ps/products/108/ab108834/documents/ab108834%20Factor%20XI%20Human%20ELISA_v3%20%28Website%29.pdf), il est conseillé d'effectuer 2 ou 3 séries de 7 points de standards...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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