サンプル | N | 平均値 | SD | CV% |
---|---|---|---|---|
Overall | 4.5% |
サンプル | N | 平均値 | SD | CV% |
---|---|---|---|---|
Overall | 9.8% |
96
%
Abcam’s Factor XI Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor XI levels in plasma, serum, and cell culture supernatants.
A Factor XI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor XI specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor XI captured in plate.
内容 | 1 x 96 tests |
---|---|
100X Streptavidin-Peroxidase Conjugate | 1 x 80µl |
10X Diluent M Concentrate | 1 x 30ml |
20X Wash Buffer Concentrate | 2 x 30ml |
50X Biotinylated Human Factor XI Antibody | 1 x 120µl |
Chromogen Substrate | 1 x 8ml |
Factor XI Microplate (12 x 8 well strips) | 1 unit |
Factor XI Standard | 2 vials |
Sealing Tapes | 3 units |
Stop Solution | 1 x 12ml |
Our Abpromise guarantee covers the use of ab108834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Sandwich ELISA | Use at an assay dependent dilution. |
ab108834 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"