製品の概要
- 製品名Human Factor XI ELISA Kit
Factor XI キット 製品一覧 - 検出方法Colorimetric
- 再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 5% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 7% - サンプルの種類Cell culture supernatant, Serum, Plasma
- アッセイタイプSandwich (quantitative)
- 検出感度= 1.5 ng/ml
- 検出範囲1.56 ng/ml - 50 ng/ml
- 添加回収試験
97.5 %
- 全工程の試験時間4h 00m
- ステップMultiple steps standard assay
- 種交差性交差種: Human
- 製品の概要
Abcam’s Factor XI Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor XI levels in plasma, serum, and cell culture supernatants.
A Factor XI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor XI specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor XI captured in plate.
- アプリケーション適用あり: Sandwich ELISAmore details
- 試験プラットフォームMicroplate
製品の特性
- 保存方法Store at -20°C. Please refer to protocols.
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内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Factor XI Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Factor XI Microplate (12 x 8 well strips) 1 unit Factor XI Standard 2 vials Sealing Tapes 3 units Stop Solution 1 x 12ml - 研究分野
- 機能Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX.
- 組織特異性Isoform 2 is produced by platelets and megakaryocytes but absent from other blood cells.
- 関連疾患Factor XI deficiency (FA11D) [MIM:612416]: A hemorrhagic disease characterized by reduced levels and activity of factor XI resulting in moderate bleeding symptoms, usually occurring after trauma or surgery. Patients usually do not present spontaneous bleeding but women can present with menorrhagia. Hemorrhages are usually moderate. Note=The disease is caused by mutations affecting the gene represented in this entry.
- 配列類似性Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
Contains 4 apple domains.
Contains 1 peptidase S1 domain. - 翻訳後修飾Activated by factor XIIa (or XII), which cleaves each polypeptide after Arg-387 into the light chain, which contains the active site, and the heavy chain, which associates with high molecular weight (HMW) kininogen.
- 細胞内局在Secreted.
- Information by UniProt
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別名
- Coagulation factor XI
- Coagulation factor XIa light chain
- F11
see all -
参照データベース
- Entrez Gene: 2160 Human
- Omim: 264900 Human
- SwissProt: P03951 Human
- Unigene: 1430 Human
アプリケーション
Our Abpromise guarantee covers the use of ab108834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent dilution. |
Human Factor XI ELISA Kit 画像
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Typical Standard CurveRepresentative Standard Curve using ab108834.
プロトコール
Human Factor XI ELISA Kit (ab108834) 使用論文
ab108834 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"