Human Factor X ELISA Kit (ab108832)
Key features and details
- Sensitivity: 1.5 ng/ml
- Range: 0.781 ng/ml - 50 ng/ml
- Sample type: Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Factor X ELISA Kit
Factor X キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 3.8% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 8.8% -
サンプルの種類
Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 1.5 ng/ml -
検出範囲
0.781 ng/ml - 50 ng/ml -
添加回収試験
97.5 %
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全工程の試験時間
4h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Human Factor Xin vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor X concentrations in plasma, serum, milk, urine, saliva, and cerebrospinal fluid.
A Factor X specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor X specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor X captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Biotinylated Antibody 1 x 60µl 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml Chromogen Substrate 1 x 7ml Factor X Microplate (12 x 8 well strips) 1 unit Factor X Standard 1 x 65ng Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. -
組織特異性
Plasma; synthesized in the liver. -
関連疾患
Defects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis. -
配列類似性
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
翻訳後修飾
The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
N- and O-glycosylated.
The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Activated factor Xa heavy chain
- Coagulation factor X
- F10
see all -
参照データベース
- Entrez Gene: 2159 Human
- Omim: 227600 Human
- SwissProt: P00742 Human
- Unigene: 361463 Human
画像
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Standard curve with background signal subtracted (duplicates; +/- SD).
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Factor X measured in undiluted culture supernatants with MCF7 levels below level of detection (duplicates +/- SD).
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Factor X measured in biological fluids (duplicates +/- SD). Serum and plasma were tested at 1/300-1/3000; other samples at 1/1-1/30.
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Representative Standard Curve using ab108832
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab108832 は 2 報の論文で使用されています。
- Feser CJ et al. Engineering CRISPR/Cas9 for Multiplexed Recombinant Coagulation Factor Production. Int J Mol Sci 23:N/A (2022). PubMed: 35563479
- Cohen CT et al. Production and control of coagulation proteins for factor X activation in human endothelial cells and fibroblasts. Sci Rep 10:2005 (2020). PubMed: 32029851