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|Serum||99||95% - 106%|
|Heparin Plasma||101||97% - 107%|
|EDTA Plasma||108||96% - 126%|
|Citrate Plasma||105||95% - 118%|
Factor VII in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor VII protein in Human serum and plasma samples.
The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Factor VII is a plasma serine protease involved in the coagulation cascade. Processed in the liver, it circulates in the bloodstream as a zymogen. During coagulation it is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin. Factor VIIa can then convert factor IX to IXa in the presence of tissue factor and calcium.
|内容||1 x 96 tests|
|10X Factor VII Capture Antibody||1 x 600µl|
|10X Factor VII Detector Antibody||1 x 600µl|
|10X Wash Buffer PT (ab206977)||1 x 20ml|
|ab221825 - Antibody Diluent 5BI||1 x 6ml|
|Factor VII Human Lyophilized Purified Protein||2 vials|
|Plate Seals||1 unit|
|Sample Diluent NS (ab193972)||1 x 50ml|
|SimpleStep Pre-Coated 96-Well Microplate (ab206978)||1 unit|
|Stop Solution||1 x 12ml|
|TMB Substrate||1 x 12ml|
Our Abpromise guarantee covers the use of ab190810 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab190810 has not yet been referenced specifically in any publications.