製品の概要

  • 製品名
    Human Factor VII ELISA Kit
    Factor VII キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 5 3.2%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 3 3.8%
  • サンプルの種類
    Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    292 pg/ml
  • 検出範囲
    0.315 ng/ml - 20 ng/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 99 95% - 106%
    Heparin Plasma 101 97% - 107%
    EDTA Plasma 108 96% - 126%
    Citrate Plasma 105 95% - 118%

  • 全工程の試験時間
    1h 30m
  • ステップ
    One step assay
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat, Goat, Guinea pig, Hamster, Cow, Dog, Pig, Arabidopsis suecica
  • 製品の概要

    Factor VII in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor VII protein in Human serum and plasma samples.


    The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • 特記事項

    Factor VII is a plasma serine protease involved in the coagulation cascade. Processed in the liver, it circulates in the bloodstream as a zymogen. During coagulation it is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin. Factor VIIa can then convert factor IX to IXa in the presence of tissue factor and calcium.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    10X Factor VII Capture Antibody 1 x 600µl
    10X Factor VII Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    ab221825 - Antibody Diluent 5BI 1 x 6ml
    Factor VII Human Lyophilized Purified Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Substrate 1 x 12ml
  • 研究分野
  • 機能
    Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
  • 組織特異性
    Plasma.
  • 関連疾患
    Factor VII deficiency
  • 配列類似性
    Belongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • 翻訳後修飾
    The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
    O- and N-glycosylated. N-glycosylation at Asn-205 occurs cotranslationally and is mediated by STT3A-containing complexes, while glycosylation at Asn-382 is post-translational and is mediated STT3B-containing complexes before folding. O-fucosylated by POFUT1 on a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 別名
    • Cf7
    • Coagulation factor VII
    • Coagulation factor VII (serum prothrombin conversion accelerator)
    • Eptacog alfa
    • F7
    • F7 deficiency
    • FA7_HUMAN
    • Factor VII deficiency
    • Factor VII heavy chain
    • FVII
    • FVII coagulation protein
    • Hypoproconvertinemia
    • Proconvertin
    • Serum prothrombin conversion accelerator
    • SPCA
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab190810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

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  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • The mean factor VII concentration was determined to be 363 ng/mL with a range of 148-616 ng/mL.

プロトコール

参考文献

ab190810 has not yet been referenced specifically in any publications.

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