製品の概要

  • 製品名Human Factor VII ELISA Kit
    Factor VII キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 8.6%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Saliva, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 6 ng/ml
  • 検出範囲
    6.25 ng/ml - 200 ng/ml
  • 添加回収試験

    97 %

  • 全工程の試験時間
    4h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Factor VII Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor VII concentrations in Human cell culture supernatants, serum and plasma.

    A Factor VII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor VII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor VII captured in plate. This assay recognizes FVII and FVIIa.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
  • 組織特異性Plasma.
  • 関連疾患Factor VII deficiency
  • 配列類似性Belongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • 翻訳後修飾The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
    O- and N-glycosylated. N-glycosylation at Asn-205 occurs cotranslationally and is mediated by STT3A-containing complexes, while glycosylation at Asn-382 is post-translational and is mediated STT3B-containing complexes before folding. O-fucosylated by POFUT1 on a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • Cf7
    • Coagulation factor VII
    • Coagulation factor VII (serum prothrombin conversion accelerator)
    • Eptacog alfa
    • F7
    • F7 deficiency
    • FA7_HUMAN
    • Factor VII deficiency
    • Factor VII heavy chain
    • FVII
    • FVII coagulation protein
    • Hypoproconvertinemia
    • Proconvertin
    • Serum prothrombin conversion accelerator
    • SPCA
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Factor VII ELISA Kit 画像

  • Representative Standard Curve using ab108829.

プロトコール

Human Factor VII ELISA Kit (ab108829) 使用論文

ab108829 has not yet been referenced specifically in any publications.

Product Wall

1. The standard provided in the kit is purified factor VII. No activation is necessary for this standard.
2. The antibodies used in this kit have not been mapped. The epitopes are unknown.

Thank you for your enquiry.

I can confirm this kit has not been specifically tested on supernatant from hepatocytes so the dilution will need to be individually optimized. Each cell culture sample is different. so it is not possible to sugges...

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Thank you for your enquiry.

Unfortunately, the kit has no cross-reactivity against mouse. Please refer page 15 of the protocol booklet on the datasheet of ab108829.

I hope this information will still be helpful. If there is anythi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"