製品の概要

  • 製品名Human Factor D ELISA Kit
    Factor D キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 4 pg/ml
  • 添加回収試験

    < 130 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 126.4 115% - 133%
    Serum 130.3 118% - 139%
    Plasma 131.1 107% - 140%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Human Factor D ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Factor D in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Factor D coated on a 96-well plate. Standards and samples are pipetted into the wells and Factor D present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Factor D antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Factor D bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimisation may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
  • 関連疾患Complement factor D deficiency (CFDD) [MIM:613912]: An immunologic disorder characterized by increased susceptibility to bacterial infections, particularly Neisseria infections, due to a defect in the alternative complement pathway. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • 配列類似性Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • Adipsin
    • ADN
    • C3 convertase activator
    • CFAD_HUMAN
    • CFD
    • Complement factor D
    • Complement factor D preproprotein
    • D component of complement
    • DF
    • PFD
    • Properdin factor D
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab99969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Factor D ELISA Kit 画像

  • Representative Standard Curve using ab99969.

  • Representative Standard Curve using ab99969.

プロトコール

Human Factor D ELISA Kit (ab99969) 使用論文

ab99969 has not yet been referenced specifically in any publications.

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