Human Estradiol E2 ELISA Kit (ab108640)

製品の概要

  • 製品名Human Estradiol E2 ELISA Kit
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    13 pg/mL 24 3 24.1%
    73 pg/mL 24 7 10.3%
    247 pg/mL 24 10 4.1%
    633 pg/mL 24 31 4.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    14 pg/mL 20 4 26.7%
    82 pg/mL 20 8 10.3%
    264 pg/mL 20 17 6.4%
    691 pg/mL 20 45 6.6%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Serum
  • アッセイタイプCompetitive
  • 検出感度
    10 pg/ml
  • 検出範囲
    10 pg/ml - 1000 pg/ml
  • 添加回収試験

    101.3 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 101.3 18pg/ml - 810pg/ml

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Estradiol E2 in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Estradiol E2 in Human serum.

    A 96-well plate has been pre-coated with goat anti-rabbit IgG. Samples, standards and the rabbit anti-Estradiol E2 Reagent and Estradiol E2-HRP conjugate are added to the wells, where Estradiol E2 in the sample competes with the added Estradiol E2-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estradiol E2 in the sample and the intensity is measured at 450 nm.

  • アプリケーション適用あり: Competitive ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab108640 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Competitive ELISA Use at an assay dependent concentration.

    Human Estradiol E2 ELISA Kit 画像

    • Representative standard curve using ab108640

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    Human Estradiol E2 ELISA Kit (ab108640) 使用論文

    ab108640 has not yet been referenced specifically in any publications.

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    ab108640 reviews

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    We purchased this ELISA kit to screen aromatase inhibitor using cultured MCF-7 cells (ER-positive breast cancer cell line). Good amount of reagents are provided in easy-to-handle vials. Good data obtained with both trials.
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    Abcam user community

    Verified customer

    投稿 Dec 17 2014

    Thank you for your enquiry. I am sorry to confirm that as far as we are aware, these kits have not been tested with samples from rat. All tested and guaranteed species cross-reactivity information is stated on our datasheets, and these are updated ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"