製品の概要

  • 製品名Human Erythropoietin ELISA Kit (EPO)
    Erythropoietin (EPO) キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 8 3.4%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 8 6.2%
  • テスト
    1 x 96 test
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    0.4 mIU/ml
  • 検出範囲
    1.6 mIU/ml - 100 mIU/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 81 71% - 93%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Erythropoietin (EPO) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Erythropoietin concentrations in cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

    Erythropoietin specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated Erythropoietin detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and unbound conjugates are then washed away using was buffer. TMB is then added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Erythropoietin captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.
  • 組織特異性Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
  • 関連疾患Genetic variation in EPO is associated with susceptbility to microvascular complications of diabetes type 2 (MVCD2) [MIM:612623]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • 配列類似性Belongs to the EPO/TPO family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • EP
    • EPO
    • EPO_HUMAN
    • Epoetin
    • Erythropoietin
    • MVCD2
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Erythropoietin ELISA Kit (EPO) 画像

  • Human Erythropoietin standard curve: mean of duplicates (+/- SD)
  • Human Erythropoietin measured in biological fluids showing quantity (mIU) per mL of tested sample. Samples diluted 1-4 fold.

  • Representative Standard Curve using ab119522.

プロトコール

Human Erythropoietin ELISA Kit (EPO) (ab119522) 使用論文

This product has been referenced in:
  • Hansen HG  et al. Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells. Sci Rep 5:18016 (2015). WB . Read more (PubMed: 26657798) »

See 1 Publication for this product

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Thank you for your enquiry regarding ab119522.

This product has been designed to detect human Erythropoietin (EPO) and it has only been tested on human samples; we do not have any information regarding its cross-reactivity with other species....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"