Human Anti-Entamoeba histolytica IgG ELISA Kit (ab108734)

製品の概要

  • 製品名Human Anti-Entamoeba histolytica IgG ELISA Kit
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Pos.Serum 7 2.1%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Pos.Serum 6 3.9%
  • テスト
    1 x 96 test
  • サンプルの種類
    Serum, Plasma
  • アッセイタイプIndirect
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s anti-Entamoeba histolytica IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Entamoeba histolytica in Human serum and plasma.

     

    A 96-well plate has been precoated with Entamoeba histolytica antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a Protein A peroxidase labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Entamoeba histolytica-specific antibodies. TMB is then catalyzed by the Protein A to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Entamoeba histolytica IgG sample captured in plate.

  • アプリケーション適用あり: Indirect ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 保存方法Store at +4°C. Please refer to protocols.
  • 内容 ラベル 1 x 96 tests
    20X Washing Solution White cap 1 x 50ml
    Cover foil 1 unit
    Entamoeba histolytica (IgG) Cut-off Control colored yellow; green cap 1 x 3ml
    Entamoeba histolytica (IgG) Negative Control colored yellow; blue cap 1 x 2ml
    Entamoeba histolytica (IgG) Positive Control colored yellow; red cap 1 x 2ml
    Entamoeba histolytica Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit
    Entamoeba histolytica Protein A Conjugate colored blue; black cap 1 x 20ml
    IgG Sample Diluent colored yellow; white cap 1 x 100ml
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution Yellow cap 1 x 15ml
  • 研究分野

    アプリケーション

    Our Abpromise guarantee covers the use of ab108734 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Indirect ELISA Use at an assay dependent dilution.

    プロトコール

    Human Anti-Entamoeba histolytica IgG ELISA Kit (ab108734) 使用論文

    ab108734 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"