製品の概要

  • 製品名Human Entactin ELISA Kit (NID1)
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 0.1 ng/ml
  • 検出範囲
    0.123 ng/ml - 30 ng/ml
  • 添加回収試験

    > 100 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 125.7 111% - 138%
    Serum 132.3 123% - 145%
    Plasma 131.4 122% - 147%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Entactin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Entactin in serum, plasma, cell culture supernatants.

    This assay employs an antibody specific for Human Entactin coated on a 96-well plate. Standards and samples are pipetted into the wells and Entactin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Entactin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Entactin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
  • 配列類似性Contains 6 EGF-like domains.
    Contains 4 LDL-receptor class B repeats.
    Contains 1 NIDO domain.
    Contains 1 nidogen G2 beta-barrel domain.
    Contains 1 thyroglobulin type-1 domain.
  • 翻訳後修飾N- and O-glycosylated.
  • 細胞内局在Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • 別名
    • Entactin
    • NID-1
    • Nid1
    • NID1_HUMAN
    • Nidogen-1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab113338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Entactin ELISA Kit (NID1) 画像

  • Representative Standard Curve using ab113338

プロトコール

Human Entactin ELISA Kit (NID1) (ab113338) 使用論文

ab113338 has not yet been referenced specifically in any publications.

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