Human ENT1 peptide (ab48606)
Key features and details
- Purity: > 90% HPLC
- Suitable for: Blocking
製品の詳細
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製品名
Human ENT1 peptide -
精製度
> 90 % HPLC. -
アクセッション番号
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Animal free
No -
由来
Synthetic -
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生物種
Human
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特性
Our Abpromise guarantee covers the use of ab48606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Blocking
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製品の状態
Liquid -
備考
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use. -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
関連情報
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別名
- Equilibrative NBMPR-sensitive nucleoside transporter
- equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter
- Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter
see all -
機能
Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs). -
組織特異性
Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen. -
配列類似性
Belongs to the SLC29A transporter family. -
翻訳後修飾
Glycosylated. -
細胞内局在
Basolateral cell membrane. Apical cell membrane. Predominantly localized in the basolateral membrane in polarised MDCK cells. - Information by UniProt
画像
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All lanes : Anti-ENT1 antibody (ab48607) at 2 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human brain tissue lysate - total protein (ab29466)
Lane 3 : Human heart tissue lysate - total protein (ab29431) withHuman ENT1 peptide (ab48606) at 2 µg/ml
Lane 4 : Human brain tissue lysate - total protein (ab29466) withHuman ENT1 peptide (ab48606) at 2 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Exposure time: 4 minutes
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab48606 は論文での使用が確認できていません。