製品の概要

  • 製品名
    Human Endostatin ELISA Kit
    Endostatin キット 製品一覧
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 10 pg/ml
  • 検出範囲
    13.7 pg/ml - 10000 pg/ml
  • 添加回収試験

    > 90 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 95.32 84% - 108%
    Serum 88.47 78% - 102%
    Plasma 92.31 80% - 104%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Human Endostatin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Endostatin in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human Endostatin coated on a 96-well plate. Standards and samples are pipetted into the wells and Endostatin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Endostatin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Endostatin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get results in 90 minutes with Human Endostatin ELISA Kit (ab184864) from our SimpleStep ELISA® range.

  • 特記事項

    Optimisation may be required with urine samples.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Detection Antibody Endostatin 2 units
    Endostatin Microplate (12 strips x 8 wells) 1 unit
    Endostatin Standard Human(recombinant) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • 研究分野
  • 関連性
    Endostatin (20-22 kDa), a cleaved product of the C-terminal domain of collagen XVIII, is an endogenous angiogenesis inhibitor. It was originally found in conditioned media from a murine endothelial tumor cell line, hemangioendothelioma. Endostatin inhibits endothelial cell migration (in vivo and in vitro) and induces endothelial cell apoptosis. It inhibits tumor growth and impairs blood vessel maturation in wound healing. Endostatin has an important role in endothelial cell adhesion and cytoskeletal organization. Endostatin can be found in vessel walls (elastic fibers) and basement membranes.
  • 別名
    • Alpha 1 collagen type 18 (XVIII)(COL18A1)
    • Alpha 1 type XVIII collagen
    • Antiangiogenic agent
    • COL18A1
    • Collagen alpha 1(XVIII) chain
    • Collagen type XVIII alpha 1
    • FLJ27325
    • FLJ34914
    • KNO
    • KNO1
    • MGC74745
    • Multi functional protein MFP
    • OTTMUSP00000023764
    • OTTMUSP00000023766
    • Procollagen type XVIII alpha 1
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab100508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab100508

  • Representative Standard Curve using ab100508

プロトコール

参考文献

ab100508 has not yet been referenced specifically in any publications.

レビューと Q&A

Both diluents should be clear. Salts, proteins, and/or other solutes may precipitate out after long periods of storage but bringing the solution up to room temperature and mixing well should re-solubilize any aggregates.


In regards to y...

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It is not necessary to dilute samples - especially if the endostatin levels are already very low. The main purpose of diluting your samples is to make sure that your sample OD falls within the OD range of your standard curve so you can accurately ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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