製品の概要

  • 製品名Human Endostatin ELISA Kit
    Endostatin キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 10 pg/ml
  • 検出範囲
    13.7 pg/ml - 10000 pg/ml
  • 添加回収試験

    > 90 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 95.32 84% - 108%
    Serum 88.47 78% - 102%
    Plasma 92.31 80% - 104%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Human Endostatin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Endostatin in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Endostatin coated on a 96-well plate. Standards and samples are pipetted into the wells and Endostatin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Endostatin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Endostatin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimisation may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性Endostatin (20-22 kDa), a cleaved product of the C-terminal domain of collagen XVIII, is an endogenous angiogenesis inhibitor. It was originally found in conditioned media from a murine endothelial tumor cell line, hemangioendothelioma. Endostatin inhibits endothelial cell migration (in vivo and in vitro) and induces endothelial cell apoptosis. It inhibits tumor growth and impairs blood vessel maturation in wound healing. Endostatin has an important role in endothelial cell adhesion and cytoskeletal organization. Endostatin can be found in vessel walls (elastic fibers) and basement membranes.
  • 別名
    • Alpha 1 collagen type 18 (XVIII)(COL18A1)
    • Alpha 1 type XVIII collagen
    • Antiangiogenic agent
    • COL18A1
    • Collagen alpha 1(XVIII) chain
    • Collagen type XVIII alpha 1
    • FLJ27325
    • FLJ34914
    • KNO
    • KNO1
    • MGC74745
    • Multi functional protein MFP
    • OTTMUSP00000023764
    • OTTMUSP00000023766
    • Procollagen type XVIII alpha 1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Endostatin ELISA Kit 画像

  • Representative Standard Curve using ab100508

  • Representative Standard Curve using ab100508

プロトコール

Human Endostatin ELISA Kit (ab100508) 使用論文

ab100508 has not yet been referenced specifically in any publications.

Product Wall

Both diluents should be clear. Salts, proteins, and/or other solutes may precipitate out after long periods of storage but bringing the solution up to room temperature and mixing well should re-solubilize any aggregates.


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It is not necessary to dilute samples - especially if the endostatin levels are already very low. The main purpose of diluting your samples is to make sure that your sample OD falls within the OD range of your standard curve so you can accurately ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"