Human Elafin ELISA Kit (Skalp) (ab100658)
Key features and details
- Sensitivity: 5 pg/ml
- Range: 6.86 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Elafin ELISA Kit (Skalp)
Elafin/ESI キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 5 pg/ml -
検出範囲
6.86 pg/ml - 5000 pg/ml -
添加回収試験
> 100 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 111.8 102% - 121% Serum 115.7 105% - 125% Plasma 112.5 103% - 122% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Elafin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Elafin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Elafin coated on a 96-well plate. Standards and samples are pipetted into the wells and Elafin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Elafin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Elafin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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特記事項
Optimization may be required with urine samples.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated anti-Human Elafin 2 vials Elafin Microplate (12 x 8 wells) 1 unit Recombinant Human Elafin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. -
配列類似性
Contains 1 WAP domain. -
ドメイン
Consists of two domains: the transglutaminase substrate domain (cementoin moiety) and the elastase inhibitor domain. The transglutaminase substrate domain serves as an anchor to localize elafin covalently to specific sites on extracellular matrix proteins. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Cementoin
- ELAF_HUMAN
- Elafin
see all -
参照データベース
- Entrez Gene: 5266 Human
- Omim: 182257 Human
- SwissProt: P19957 Human
- Unigene: 112341 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab100658 は 3 報の論文で使用されています。
- Olmos-Ortiz A et al. Compartmentalized Innate Immune Response of Human Fetal Membranes against Escherichia coli Choriodecidual Infection. Int J Mol Sci 23:N/A (2022). PubMed: 35328414
- Tehrani FA et al. Induction of antimicrobial peptides secretion by IL-1ß enhances human amniotic membrane for regenerative medicine. Sci Rep 7:17022 (2017). ELISA . PubMed: 29208979
- Tehrani FA et al. The effects of preservation procedures on antibacterial property of amniotic membrane. Cryobiology 67:293-8 (2013). PubMed: 23988559