Human Dry Eye Disease 抗体 Array (40 Targets)- Quantitative (ab197444)
製品の概要
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製品名
Human Dry Eye Disease Antibody Array (40 Targets)- Quantitative -
サンプルの種類
Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate -
アッセイタイプ
Quantitative -
全工程の試験時間
6h 00m -
種交差性
交差種: Human -
製品の概要
ab197444 can be used for quantitative measurement of 40 Human Dry Eye Disease associated cytokines. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.
Targets names: EGF, EGF R, Eotaxin, G-CSF, GRO, HB-EGF, ICAM-1, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-6R, IL-7, IL-8, IL-10, IL-11, IL-12p40, IL-12p70, IL-13, IL-15, IL-16, IL-17, IP-10, Lipocalin-2, MCP-1, MIG, MIP-1α, MIP-1β, MIP-1δ, MMP-9, RANTES, TGFβ1, TIMP-1, TIMP-2, TNFα, VEGF.
Learn more about quantitative antibody arrays
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特記事項
Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins.
Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc.
For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation.
Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader.
The standard is affinity purified.
Learn more about quantitative antibody arrays
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 50 tests 22 tests 8 tests 20X Wash Buffer I 6 x 30ml 3 x 30ml 2 x 30ml 20X Wash Buffer II 2 x 30ml 1 x 30ml 1 x 30ml Cy3 equivalent dye-conjugated Streptavidin 4 x 5µl 2 x 5µl 1 x 5µl Human Dry Eye Disease Array (40 T) Biotinylated Antibody Cocktail 4 units 2 units 1 unit Human Dry Eye Disease Array (40 T) Glass Slide 4 slides 2 slides 1 slide Human Dry Eye Disease Array (40 T) Lyophilized Cytokine Standard Mix 2 vials 2 vials 1 vial Sample Diluent 2 x 15ml 1 x 15ml 1 x 15ml Slide Washer/Dryer (30 mL Centrifuge Tube) 2 units 1 unit 1 unit
画像
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Assay summary for Abcam Human Dry Eye Disease Antibody Array (40 targets) - Quantitative (ab197444).
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Array Map Abcam Human Dry Eye Disease Antibody Array (40 targets) - Quantitative (ab197444).
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Standard Curve obtained using Human Dry Eye Disease Antibody Array (40 targets) - Quantitative (ab197444).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab197444 は論文での使用が確認できていません。