製品の概要

  • 製品名Human DR5 ELISA Kit (CD262)
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Sample A 8 483pg/ml 7 1.39%
    Sample B 8 117pg/ml 4 3.23%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Sample A 9 679pg/ml 45 6.58%
    Sample B 9 186pg/ml 14 7.44%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Cell culture extracts
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 6 pg/ml
  • 検出範囲
    31.25 pg/ml - 1000 pg/ml
  • 全工程の試験時間
    2h 30m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s DR5 (CD262) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of DR5 in Human serum, plasma, buffered solutions and cell culture media.

    A monoclonal antibody specific for DR5 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known DR5 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for DR5 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of DR5 present in the samples. 

    This kit will recognize both endogenous and recombinant Human DR5.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 関連性DR5 is a member of the TNF-receptor superfamily, and contains an intracelluar death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein.
  • 細胞内局在Cell Membrane; single-pass type I membrane protein.
  • 別名
    • Death receptor 5
    • KILLER
    • TNFRSF10B
    • TRAIL R2
    • TRAIL receptor 2
    • TRICK2
    • TRICKB
    • ZTNFR9
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab46013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human DR5 ELISA Kit (CD262) 画像

  • Representative Standard Curve using ab46013

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Human DR5 ELISA Kit (CD262) (ab46013) 使用論文

ab46013 has not yet been referenced specifically in any publications.

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