製品の概要

  • 製品名Human DR4 ELISA Kit (CD261)
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    A 8 475pg/ml 13 2.76%
    B 8 54pg/ml 4 7.57%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    A 12 498pg/ml 40 8.11%
    B 12 53pg/ml 7 13.87%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 8 pg/ml
  • 検出範囲
    31.25 pg/ml - 1000 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 93.4 250pg/ml - 1000pg/ml

  • 全工程の試験時間
    2h 30m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s DR4 (CD261) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of DR4 in serum, plasma, buffered solutions and cell culture media.

    A monoclonal antibody specific for DR4 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known DR4 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for DR4 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of DR4 present in the samples.

    This kit will recognize both endogenous and recombinant Human DR4.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性Human Trail R1, CD261 or DR4 (Death Receptor 4) is a TNF receptor, which is a receptor for Trail (APO2 ligand). DR4 is involved in apoptosis. In the Trail receptor family, DR4, but also Trail R2 (DR5) transduce an apoptosis signal, whereas Trail R3 (DcR1) and Trail R4 (DcR2) antagonize TRAIL-induced apoptosis. Binding of trimeric Trail to DR4 induces apoptosis by oligomerization (likely to be trimerization) of the receptor. DR4 cDNA encodes a 468 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. DR4 shares 55% of its amino acid sequence with Trail R2. DR4 is widely expressed in normal tissue.
  • 細胞内局在Cell Membrane; single-pass type I membrane protein
  • 別名
    • APO2
    • CD261
    • Death receptor 4
    • TNFRSF10A
    • TRAIL receptor 1
    • TRAILR1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab46510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human DR4 ELISA Kit (CD261) 画像

  • Representative Standard Curve using ab46510

プロトコール

Human DR4 ELISA Kit (CD261) (ab46510) 使用論文

ab46510 has not yet been referenced specifically in any publications.

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A standard lysis buffer (such as RIPA buffer) can be used to prepare the cell lysates and the use protease inhibitor is indeed recommended.

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