Human DR4 ELISA Kit (CD261) (ab46510)
Key features and details
- Sensitivity: 8 pg/ml
- Range: 31.25 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human DR4 ELISA Kit (CD261) -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% A 8 475pg/ml 13 2.76% B 8 54pg/ml 4 7.57% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% A 12 498pg/ml 40 8.11% B 12 53pg/ml 7 13.87% -
サンプルの種類
Cell culture supernatant, Serum, Cell Lysate -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 8 pg/ml -
検出範囲
31.25 pg/ml - 1000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 93.4 250pg/ml - 1000pg/ml -
全工程の試験時間
2h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s DR4 (CD261) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of DR4 in serum, plasma, buffered solutions and cell culture media.
A monoclonal antibody specific for DR4 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known DR4 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for DR4 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of DR4 present in the samples.
This kit will recognize both endogenous and recombinant Human DR4.
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特記事項
The standard is affinity purified.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 2 x 96 tests 1 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 15ml 200X Wash Buffer White 2 x 10ml 1 x 10ml Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7ml Biotinylated anti-DR4 Red 2 x 400µl 1 x 400µl Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml DR4 Microplate (12 x 8 well strips) 2 units 1 unit DR4 Standard (lyophilized) Yellow 4 vials 2 vials HRP Diluent Red 1 x 23ml 1 x 12ml Stop Reagent Black 2 x 11ml 1 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl -
研究分野
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関連性
Human Trail R1, CD261 or DR4 (Death Receptor 4) is a TNF receptor, which is a receptor for Trail (APO2 ligand). DR4 is involved in apoptosis. In the Trail receptor family, DR4, but also Trail R2 (DR5) transduce an apoptosis signal, whereas Trail R3 (DcR1) and Trail R4 (DcR2) antagonize TRAIL-induced apoptosis. Binding of trimeric Trail to DR4 induces apoptosis by oligomerization (likely to be trimerization) of the receptor. DR4 cDNA encodes a 468 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. DR4 shares 55% of its amino acid sequence with Trail R2. DR4 is widely expressed in normal tissue. -
細胞内局在
Cell Membrane; single-pass type I membrane protein -
別名
- APO2
- CD261
- Death receptor 4
see all -
参照データベース
- Entrez Gene: 8797 Human
- SwissProt: O00220 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab46510 は論文での使用が確認できていません。