Human DNA PKcs (phospho S2056) peptide (ab20406)



  • 由来Synthetic
  • アミノ酸配列
    • アクセッション番号P78527
    • 生物種Human
    • 修飾phospho S2056
    • 配列の追加情報This peptide can be used in studies using ab18192 and ab20407


Our Abpromise guarantee covers the use of ab20406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Blocking - Blocking peptide for Anti-DNA PKcs (phospho S2056) antibody - ChIP Grade (ab18192)

  • 製品の状態Liquid
  • 備考

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...


  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.


  • 別名
    • DNA dependent protein kinase catalytic subunit
    • DNA PK catalytic subunit
    • DNA-dependent protein kinase catalytic subunit
    • DNA-PK catalytic subunit
    • DNA-PKcs
    • DNAPK
    • DNAPK catalytic subunit
    • DNPK 1
    • DNPK1
    • Hyper radiosensitivity of murine scid mutation, complementing 1
    • Hyperradiosensitivity complementing 1, mouse, homolog of 1
    • HYRC
    • HYRC 1
    • HYRC1
    • IMD26
    • p350
    • p460
    • PKRDC
    • PRKDC
    • Protein Kinase DNA Activated Catalytic Polypeptide
    • XRCC 7
    • XRCC7
    see all
  • 機能Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.
  • 配列類似性Belongs to the PI3/PI4-kinase family.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 2 HEAT repeats.
    Contains 1 PI3K/PI4K domain.
    Contains 3 TPR repeats.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR. Autophosphorylated on Thr-2609, Thr-2638 and Thr-2647. Thr-2609 is a DNA damage-inducible phosphorylation site (inducible with ionizing radiation, IR). Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair.
    S-nitrosylated by GAPDH.
  • 細胞内局在Nucleus.
  • Information by UniProt

Human DNA PKcs (phospho S2056) peptide 画像

  • All lanes : Anti-DNA PKcs (phospho S2056) antibody - ChIP Grade (ab18192) at 1 µg/ml

    Lane 1 : HeLa Gamma Irradiated Whole Cell Lysate Pack (ab13823) at 20 µg
    Lane 2 : 20ug untreated HeLa cell extract
    Lane 3 : HeLa Gamma Irradiated Whole Cell Lysate Pack (ab13823) at 20 µg with Human DNA PKcs (phospho S2056) peptide (ab20406) at 1 µg/ml
    Lane 4 : 20ug untreated HeLa cell extract with Human DNA PKcs (phospho S2056) peptide (ab20406) at 1 µg/ml
    Lane 5 : HeLa Gamma Irradiated Whole Cell Lysate Pack (ab13823) at 20 µg with Human DNA PKcs peptide (ab20407) at 1 µg/ml
    Lane 6 : 20ug untreated HeLa cell extract with Human DNA PKcs peptide (ab20407) at 1 µg/ml

    Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution

    Performed under reducing conditions.

    Observed band size : 460 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 270 kDa (possible cleavage fragment,cross reactivity).

Human DNA PKcs (phospho S2056) peptide (ab20406) 使用論文

ab20406 has not yet been referenced specifically in any publications.

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