Human DKK1 ELISA Kit (Dickkopf-1) (ab100501)

製品の概要

  • 製品名Human DKK1 ELISA Kit (Dickkopf-1)
    DKK1 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 100 pg/ml
  • 検出範囲
    122.9 pg/ml - 30000 pg/ml
  • 添加回収試験

    90 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 108.3 93% - 123%
    Serum 92.3 62% - 130%
    Plasma 72.8 59% - 110%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s DKK1 (Dickkopf-1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human DKK1 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human DKK1 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human DKK1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性DKK1 is an inhibitor of the Wnt signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
  • 別名
    • Dickkopf 1
    • Dickkopf homolog 1
    • Dickkopf related protein 1
    • DKK 1
    • hDkk 1
    • hDkk1
    • mDkk1
    • SK
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human DKK1 ELISA Kit (Dickkopf-1) 画像

  • Representative Standard Curve using ab100501.

  • Representative Standard Curve using ab100501.

プロトコール

Human DKK1 ELISA Kit (Dickkopf-1) (ab100501) 使用論文

ab100501 has not yet been referenced specifically in any publications.

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Thank you for your interest in our kits. I will send the product inserts first thing tomorrow morning, EST. The kits will be compatible with the automated ELISA machine, if the automated set-up approximates the conditions specified in the kit protocols...

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This kit has been shown that to cross-react with mouse samples, however, we do not have any information about cross-reactivity with rabbit samples.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"