製品の概要

  • 製品名Human Decorin ELISA Kit (DCN)
    Decorin キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 1.5 pg/ml
  • 検出範囲
    0.96 pg/ml - 700 pg/ml
  • 添加回収試験

    > 93 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 93.82 84% - 104%
    Serum 122.5 111% - 130%
    Plasma 111.8 103% - 119%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Decorin (DCN) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Decorin in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Decorin coated on a 96-well plate. Standards and samples are pipetted into the wells and Decorin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti Human Decorin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Decorin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimisation may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能May affect the rate of fibrils formation.
  • 関連疾患Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048]. Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance.
  • 配列類似性Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
    Contains 12 LRR (leucine-rich) repeats.
  • 翻訳後修飾The attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin.
  • 細胞内局在Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 別名
    • Bone proteoglycan II
    • CSCD
    • DCN
    • DCN protein
    • Decorin
    • Decorin proteoglycan
    • Dermatan sulphate proteoglycans II
    • DKFZp686J19238
    • DSPG 2
    • DSPG2
    • PG 40
    • PG II
    • PG S2
    • PG-S2
    • PG40
    • PGII
    • PGS 2
    • PGS2
    • PGS2_HUMAN
    • Proteoglycan core protein
    • SLRR1B
    • Small leucine rich protein 1B
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab99998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Decorin ELISA Kit (DCN) 画像

  • DCN measured in biological fluids showing quantity (pg) per mL of tested sample

  • Representative Standard Curve using ab99998.

プロトコール

Human Decorin ELISA Kit (DCN) (ab99998) 使用論文

This product has been referenced in:
  • Ono YJ  et al. Decorin induced by progesterone plays a crucial role in suppressing endometriosis. J Endocrinol 223:203-16 (2014). Human . Read more (PubMed: 25244916) »

See 1 Publication for this product

Product Wall

Decorin ELISA kit easy to use

Good Excellent 5/5 (Ease of Use)
Abreviews
It's very easy to use.
We tested this kit on pig wounded skin tissue.
We got acceptable result but we need more samples to statistics analysis.
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投稿 May 29 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"