製品の概要

  • 製品名Human DCR2 ELISA Kit (CD264)
    DcR2 キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Sample A 8 5723pg/ml 212 3.7%
    Sample B 8 3063pg/ml 218 7.1%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Sample A 12 6531pg/ml 627 9.6%
    Sample B 12 3123pg/ml 369 11.8%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Serum, Cell culture extracts
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 64 pg/ml
  • 検出範囲
    312.5 pg/ml - 10000 pg/ml
  • 全工程の試験時間
    3h 40m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s DCR2 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of DCR2 in Human serum, plasma, buffered solutions or supernatants.

    A monoclonal antibody specific for DCR2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known DCR2 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for DCR2 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of DCR2 present in the samples.

    This kit will recognize both endogenous and recombinant Human DCR2.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 関連性DcR2 is a type 1 TNF R family membrane protein which is a receptor for TRAIL. In the Trail receptor family, DcR2 but also Trail R3 (DcR1) antagonize TRAIL-induced apoptosis whereas Trail R1 (DR4) and Trail R2 (DR5) transducer an apoptosis signal. DcR2 has a truncated intracellular domain. Overexpression of DcR2 has been shown to protect cells bearing Trail R1 and/or Trail R2 from Trail-induced apoptosis. Many tumor cell lines don’t express the decoy receptors and are therefore sensitive to TRAIL if they express Trail R1 and/or Trail R2.
  • 細胞内局在Cell Membrane; single-pass type I membrane protein
  • 別名
    • CD264
    • CD264 antigen
    • DCR2
    • Decoy receptor 2
    • TNF related apoptosis inducing ligand receptor 4
    • TRAIL R4
    • TRAIL receptor 4
    • TRAIL receptor with a truncated death domain
    • TRAILR4
    • TRUNDD
    • Tumor necrosis factor receptor superfamily member 10d decoy with truncated death domain
    • Tumor necrosis factor receptor superfamily member 10D precursor
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab46017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human DCR2 ELISA Kit (CD264) 画像

  • Representative Standard Curve using ab46017

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Human DCR2 ELISA Kit (CD264) (ab46017) 使用論文

ab46017 has not yet been referenced specifically in any publications.

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