製品の概要

  • 製品名
    Human DcR1 ELISA Kit (CD263)
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Sample A 8 7171pg/ml 192 2.7%
    Sample B 8 3584pg/ml 244 6.8%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Sample A 9 7311pg/ml 515 7%
    Sample B 9 2654pg/ml 356 9.7%
  • サンプルの種類
    Serum, Cell culture extracts
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 147 pg/ml
  • 検出範囲
    312.5 pg/ml - 10000 pg/ml
  • 全工程の試験時間
    3h 40m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s DcR1 (CD263) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of DcR1 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

    A monoclonal antibody specific for DcR1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known DcR1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for DcR1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of DcR1 present in the samples.

    This kit will recognize both endogenous and recombinant Human DcR1.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 ラベル 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml
    Biotinylated anti-DcR1 Red 1 x 400µl 2 x 400µl
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    DcR1 Microplate (12 x 8 well strips) 1 unit 2 units
    DcR1 Standard (Lyophilized) Yellow 2 vials 4 vials
    HRP Diluent Red 1 x 23ml 1 x 23ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • 研究分野
  • 関連性
    DcR1 is a member of the TNF-receptor superfamily. It contains an extracellular TRAIL-binding domain and a transmembrane domain, but no cytoplasmic death domain. It is not capable of inducing apoptosis, and is thought to function as an antagonistic receptor that protects cells from TRAIL-induced apoptosis. This gene was found to be a p53-regulated DNA damage-inducible gene. The expression of this gene was detected in many normal tissues but not in most cancer cell lines, which may explain the specific sensitivity of cancer cells to the apoptosis-inducing activity of TRAIL.
  • 細胞内局在
    Cell membrane; lipid-anchor; GPI-anchor
  • 別名
    • CD263
    • DCR1
    • Decoy receptor 1
    • DR6
    • LIT
    • Lymphocyte inhibitor of TRAIL
    • TRAIL R3
    • TRAIL receptor 3
    • TRAILR3
    • TRID
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab46016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab46016

プロトコール

参考文献

This product has been referenced in:
  • Anand A  et al. Does DcR1 (TNF-related apoptosis-inducing-ligand Receptor 3) have any role in human AMD pathogenesis? Sci Rep 4:4114 (2014). Read more (PubMed: 24534820) »

See 1 Publication for this product

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