製品の概要

  • 製品名Human Cytochrome c ELISA Kit
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 6%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 4%
  • テスト
    1 x 96 test
  • サンプルの種類
    Cell Lysate
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    0.05 ng/ml
  • 検出範囲
    0.08 ng/ml - 5 ng/ml
  • 添加回収試験

    82 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell Lysate 98 78% - 119%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Cytochrome c Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Cytochrome c concentrations in cell lysate.

    Cytochrome c specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated Cytochrome c detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and unbound conjugates are then washed away using was buffer. TMB is then added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Cytochrome c captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
  • 細胞内局在Mitochondrion matrix
  • 別名
    • CYC
    • CYCS
    • Cytochrome c somatic
    • HCS
    • THC4
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Cytochrome c ELISA Kit 画像

  • Representative Standard Curve using ab119521.

プロトコール

Human Cytochrome c ELISA Kit (ab119521) 使用論文

ab119521 has not yet been referenced specifically in any publications.

Product Wall



The lysis buffer in kits ab119507 and ab119521 are identical, so you can analyze lysates prepared for ab119507 with ab119521.
For ab119521, the lysis buffer allows for lysis of the cellular membrane but not other compartments with in the...

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Unfortunately, we would not recommend using the Cytochrome c Human ELISA Kit (with color giving dyes), ab119521 with serum sample as when we have tried this we have observed false pos...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"