製品の概要

  • 製品名Human Cystatin C ELISA Kit
    Cystatin C キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 10 pg/ml
  • 検出範囲
    0.312 ng/ml - 20 ng/ml
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Human Cystatin C in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Cystatin C in cell culture supernatants, serum, plasma (heparin, EDTA) saliva, urine and Human milk.


    A Cystatin C specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Cystatin C is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Cystatin C amount of sample captured in plate.


    Save time with a 90-minute ELISA protocol. Try Human Cystatin C ELISA Kit (ab179883) from our SimpleStep ELISA® range.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
  • 組織特異性Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.
  • 関連疾患Defects in CST3 are the cause of amyloidosis type 6 (AMYL6) [MIM:105150]; also known as hereditary cerebral hemorrhage with amyloidosis (HCHWA), cerebral amyloid angiopathy (CAA) or cerebroarterial amyloidosis Icelandic type. AMYL6 is a hereditary generalized amyloidosis due to cystatin C amyloid deposition. Cystatin C amyloid accumulates in the walls of arteries, arterioles, and sometimes capillaries and veins of the brain, and in various organs including lymphoid tissue, spleen, salivary glands, and seminal vesicles. Amyloid deposition in the cerebral vessels results in cerebral amyloid angiopathy, cerebral hemorrhage and premature stroke. Cystatin C levels in the cerebrospinal fluid are abnormally low.
    Genetic variations in CST3 are associated with age-related macular degeneration type 11 (ARMD11) [MIM:611953]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • 配列類似性Belongs to the cystatin family.
  • 翻訳後修飾The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • ARMD11
    • bA218C14.4 (cystatin C)
    • Cst3
    • Cystatin-3
    • Cystatin-C
    • CYTC_HUMAN
    • Gamma-trace
    • Neuroendocrine basic polypeptide
    • Post-gamma-globulin
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab119589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Cystatin C ELISA Kit 画像

  • Representative Standard Curve using 119589.

プロトコール

Human Cystatin C ELISA Kit (ab119589) 使用論文

ab119589 has not yet been referenced specifically in any publications.

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