Human Cystatin C ELISA Kit (ab119589)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 0.312 ng/ml - 20 ng/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Milk, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Cystatin C ELISA Kit
Cystatin C キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% 1 16 0.687ng/ml 0.044 = 6.4% 2 16 2.837ng/ml 0.207 = 7.3% 3 16 10.374ng/ml 0.684 = 6.6% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% 1 24 0.647ng/ml 0.051 = 7.9% 2 24 2.715ng/ml 0.217 = 8% 3 24 10.112ng/ml 0.859 = 8.5% -
サンプルの種類
Cell culture supernatant, Milk, Urine, Serum, Hep Plasma, EDTA Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 10 pg/ml -
検出範囲
0.312 ng/ml - 20 ng/ml -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Human Cystatin C in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Cystatin C in cell culture supernatants, serum, plasma (heparin, EDTA) saliva, urine and Human milk.
A Cystatin C specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Cystatin C is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Cystatin C amount of sample captured in plate.
Get results in 90 minutes with Human Cystatin C ELISA Kit (ab179883) from our SimpleStep ELISA® range.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml 1 x 12ml Anti-Human Cystatin C antibody Microplate (12 x 8 wells) 1 unit 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl 1 x 100µl Biotinylated anti- Human Cystatin C antibody 1 x 100µl 1 x 100µl Lyophilized recombinant Human Cystatin C standard 2 x 20ng 2 x 20ng Plate Seal 4 units 4 units Sample Diluent Buffer Green Cap 1 x 30ml 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml 1 x 10ml Wash Buffer (25X) 1 x 20ml 1 x 20ml -
研究分野
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機能
As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. -
組織特異性
Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland. -
関連疾患
Amyloidosis 6
Macular degeneration, age-related, 11 -
配列類似性
Belongs to the cystatin family. -
翻訳後修飾
The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21. -
細胞内局在
Secreted. - Information by UniProt
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別名
- AD 8
- AD8
- Amyloid angiopathy and cerebral hemorrhage
see all -
参照データベース
- Entrez Gene: 1471 Human
- Omim: 604312 Human
- SwissProt: P01034 Human
- Unigene: 304682 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab119589 は 1 報の論文で使用されています。
- Natarajan S et al. Development and Evaluation of a Quantitative Fluorescent Lateral Flow Immunoassay for Cystatin-C, a Renal Dysfunction Biomarker. Sensors (Basel) 21:N/A (2021). PubMed: 34063596